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Plasmid#21814DepositorInsertPERK (Eif2ak3 Mouse)
UseTagsMyc (9E10)ExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET30 GLTP
Plasmid#170733PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorInsertGLTP (GLTP Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Stat3-C
Plasmid#13373PurposeRetroviral expression of dominant active mouse Stat3DepositorInsertsignal transducer and activator of transcription 3 (dominant active mutant) (Stat3 Mouse)
UseRetroviralTagsExpressionMammalianMutationChanged Alanine 661 to cysteine and asparagine 66…PromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
HA GSK3 beta K85A pcDNA3
Plasmid#14755DepositorInsertGSK3 beta K85A (GSK3B Human)
UseTagsHAExpressionMammalianMutationK85A. Kinase dead mutant.PromoterAvailable sinceApril 12, 2007AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSN818
Plasmid#190690PurposeMammalian expression plasmid of anti-kinesin (Mouse). Expresses H2 IgG (heavy chain and light chain).DepositorInsertanti-kinesin (Mus musculus) recombinant mouse monoclonal antibody; IgG heavy and light chains
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
FokI-dCas9
Plasmid#52970PurposeExpresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cellsDepositorInsertFok1 fused to dCas9
UseCRISPRTagsFok1 nuclease (homodimeric wildtype) and NLSExpressionMammalianMutationD10A and H840A in Cas9PromoterCMVAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CDK14_IDG-K
Plasmid#158172PurposeGateway destination clone of CDK14 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CDK14 (CDK14 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-DIO-mRuby2
Plasmid#104058PurposeAAV expression of Cre-dependent reporter mRuby2DepositorInsertmRuby2
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG-DIO-mTurquoise2
Plasmid#104059PurposeAAV expression of Cre-dependent reporter mTurquoise2DepositorInsertmTurquoise2
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
Kras-Src FRET biosensor
Plasmid#78302PurposeFRET biosensor. To monitor Src kinase activity in mammalian cells by FRETDepositorInsertSrc biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
plk1 T210D
Plasmid#132964PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damageDepositorInsertpolo-like kinase 1 (PLK1 Human)
UseTagsExpressionMammalianMutationT210DPromoterAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB101
Plasmid#68555PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28b-hUba2-His
Plasmid#53117Purposeexpression of full length human Uba2 with Cterminal His-tag in E.coliDepositorInsertUBA2 (UBA2 Human)
UseTagsHis and T7 tagExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pG108-K - AhpC-Glow
Plasmid#185135PurposeBacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoterDepositorInsertsGlow gene
Bacteroides thetaiotaomicron ahpC promoter
UsePg106TagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMADM-beta
Plasmid#36891DepositorInsertsBeta-Geo
thymidine kinase
UseTagsExpressionMammalianMutationPromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMSR0
Plasmid#161984PurposeAllele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection markerDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-SpySrtA
Plasmid#51139Purposeexpression plasmid for N-terminal his tagged S.pyogenes SrtADepositorInsertS.pyogenes SrtA
UseTags6x HisExpressionBacterialMutationDeleted amino acids 1-80PromoterT7Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
6x-His_A1aY1_GFP
Plasmid#158753PurposeBacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purificationDepositorInsert6xHistidine_A1aY1_GFP
UseTags6x-Histidine tag, GFP, T7 leader sequence, and Th…ExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR
Plasmid#161983PurposeAllele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile 630. Uses inducible expression of CD2517.1 toxin as a counter-selection markerDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2021AvailabilityAcademic Institutions and Nonprofits only