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PurposeRetroviral expression of dominant active mouse Stat3
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13373 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMXs
- Backbone size w/o insert (bp) 4600
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesignal transducer and activator of transcription 3 (dominant active mutant)
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Alt nameStat3-C
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Alt nameStat3
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SpeciesM. musculus (mouse)
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Insert Size (bp)2313
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MutationChanged Alanine 661 to cysteine and asparagine 663 to cysteine, dominant active mutation.
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Entrez GeneStat3 (a.k.a. 1110034C02Rik, Aprf)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pMX-S1811 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMXs-Stat3-C was a gift from Shinya Yamanaka (Addgene plasmid # 13373 ; http://n2t.net/addgene:13373 ; RRID:Addgene_13373) -
For your References section:
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi K, Yamanaka S. Cell. 2006 Aug 25. 126(4):663-76. 10.1016/j.cell.2006.07.024 PubMed 16904174