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PurposeRetroviral expression of mouse beta-catenin S33Y mutant
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13371 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMXs
- Backbone size w/o insert (bp) 4600
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecatenin (cadherin associated protein), beta 1 (S33Y mutant)
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Alt namebeta-catenin S33Y, L388P
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SpeciesM. musculus (mouse)
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Insert Size (bp)2346
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MutationSerine 33 to Tyrosine and Leucine 388 to Proline
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Entrez GeneCtnnb1 (a.k.a. Bfc, Catnb, Mesc)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pMX-S1811 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMXs-beta-catenin-S33Y was a gift from Shinya Yamanaka (Addgene plasmid # 13371 ; http://n2t.net/addgene:13371 ; RRID:Addgene_13371) -
For your References section:
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi K, Yamanaka S. Cell. 2006 Aug 25. 126(4):663-76. 10.1016/j.cell.2006.07.024 PubMed 16904174