GRK2-CAAX
(Plasmid
#166224)
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PurposeGRK2-CAAX mammalian expression plasmid
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 166224 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRK5
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Vector typeMammalian Expression
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Selectable markersn/a
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebovine GRK2-CAAX
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SpeciesB. taurus (bovine)
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Insert Size (bp)2070
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Mutationadding CAAX sequence at C terminal
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GenBank IDNM_174710 NM_174710
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Entrez GeneGRK2 (a.k.a. ADRBK1)
- Promoter CMV
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Tag
/ Fusion Protein
- no
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer SV40 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GRK2-CAAX was a gift from Robert Lefkowitz (Addgene plasmid # 166224 ; http://n2t.net/addgene:166224 ; RRID:Addgene_166224) -
For your References section:
Isoprenylation in regulation of signal transduction by G-protein-coupled receptor kinases. Inglese J, Koch WJ, Caron MG, Lefkowitz RJ. Nature. 1992 Sep 10;359(6391):147-50. doi: 10.1038/359147a0. 10.1038/359147a0 PubMed 1522899
