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Lenti-SLC35B2-sgRNA

Plasmid

#154860

Purpose

Lentiviral expression of Cas9 and sgRNA targeting SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 sgRNA (SLC35B2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET27b-ecDHFR-N23PP/L28F

Plasmid

#118584

Purpose

Bacterial expression of E.coli DHFR N23PP and L28F mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Use

Tags

Expression

Bacterial

Mutation

L28 mutated to F28, N23 mutated to P23, a proline…

Promoter

T7

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV intron 4xGCN4 Renilla FKBP STOP 24xMS2v5 SV40 CTE polyA

Plasmid

#104984

Purpose

Expresses ST-Renilla reporter in mammalian cells

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

Renilla luciferase

Use

Tags

24xMS2v5 stem loops in 3'UTR, 4xGCN4 repeats…

Expression

Bacterial and Mammalian

Mutation

Promoter

TetCMV

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1-SLC35B2-FLAG

Plasmid

#154863

Purpose

Transient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

ctcctggtgcagtacttc => ttattagtccaatatttt

Promoter

CMV

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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LacI/109-pHG165c

Plasmid

#90058

Purpose

WT LacI polymorphism with T at position 109

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LacI/109

Use

Tags

Expression

Bacterial

Mutation

109

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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DEL-pHG165a

Plasmid

#90064

Purpose

LLhG coding region on pHG165a interrupted with a single base-pair deletion, 'no repressor' control plasmid

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

DEL

Use

Tags

Expression

Bacterial

Mutation

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pTH743-CEN-RLuc/slowstaCFLuc

Plasmid

#38223

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

The last three codons of the full-length Firefly …

Promoter

ADH1 and TDH3 (=GPD)

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUBC-EBFP2

Plasmid

#212314

Purpose

Lentiviral backbone with EBFP2 reporter

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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FAB(PX-LD)

Plasmid

#214426

Purpose

Detection of peroxisome-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FKBP-V5

Use

Tags

CFAST-Halo and FKBP-V5-RspAN

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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Halotag-6xHp

Plasmid

#214414

Purpose

Lipid droplet localization of 6xHp of Spastin fused to Halotag

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

6x Spastin aa 43-92 (SPAST Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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mApple-6xHp

Plasmid

#214410

Purpose

Lipid droplet localization of 6xHp of Spastin fused to mApple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

Spastin aa 43-92 (SPAST Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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CFAST-Halotag-6xHp

Plasmid

#214416

Purpose

Lipid droplet localization of CFAST fused to Halotag-6xHp

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

deletion

Use

Tags

CFAST-Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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mApple-1xHp

Plasmid

#214409

Purpose

Lipid droplet and ER localization of 1xHp of Spastin fused to mApple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

Spastin aa 43-92 (SPAST Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-RPLP15UTR-hRluc-A60

Plasmid

#182639

Purpose

Transcription template plasmid for RPLP1 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

Depositor

Stephen Floor

Article

Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

Insert

Renilla luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

The transcription is driven by a T7 promoter, pai…

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGEMHE-bNup98-DN-NLS-mClover3

Plasmid

#171495

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged bovine Nup98 DN mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

Nup98-DN (NUP98 B. tarus (bovine))

Use

Pgehme

Tags

mClover3

Expression

Mutation

Promoter

T7

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-XYLT2-sgRNA

Plasmid

#154862

Purpose

Lentiviral expression of Cas9 and sgRNA targeting XYLT2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

XYLT2 sgRNA (XYLT2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pTH862-CEN-slowmaxRLuc/minCFLuc

Plasmid

#115371

Purpose

Expresses translation initiation-controlled Renilla luciferase and translation elongation-controlled firefly luciferase

Depositor

Tobias von der Haar

Inserts

Firefly Luciferase (codon minimised)
Gcn4 5'-UTR + Renilla luciferase (codon optimised)

Use

Tags

Expression

Yeast

Mutation

All codons have been changed for the most favoura…

Promoter

ADH1 and TDH3

Available since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

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LLhT-pHG165a V52A

Plasmid

#90050

Purpose

Constitutive expression of a chimeric LacI:TreR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhT/V52A

Use

Tags

Expression

Bacterial

Mutation

V52A

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhA-pHG165a Q55A

Plasmid

#90052

Purpose

Constitutive expression of a chimeric LacI:AscG transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhA/Q55A

Use

Tags

Expression

Bacterial

Mutation

Q55A

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhC-pHG165a Q55I

Plasmid

#90053

Purpose

Constitutive expression of a chimeric LacI:CytR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhC/Q55I

Use

Tags

Expression

Bacterial

Mutation

Q55I

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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