Addgene

Showing: 561 - 580 of 1133 results

Results per page:

Sort results by:

pHR-BTLA-mGFP

Plasmid

#180791

Purpose

Expression of human BTLA fused to mEGFP

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLA (BTLA Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mutation

Promoter

SFFV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-SNAP-PD-1

Plasmid

#223572

Purpose

Lentiviral expression of human PD-1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-1 (PDCD1 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pirespuro2-CD86-mEos2

Plasmid

#98284

Purpose

mammalian expression of CD86-mEos2

Depositor

Mike Heilemann

Article

Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

Insert

CD86 (CD86 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
SBP-GFP-Tac

Plasmid

#162505

Purpose

Expresses GFP tagged Tac in a RUSH vector in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

Tac (IL2RA Human)

Use

Tags

GFP and SBP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC_C_IGF1R

Plasmid

#187770

Purpose

MAC-tagged gene expression of human IGF1R

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

IGF1R (IGF1R Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Tac-GFP

Plasmid

#162494

Purpose

Expresses Tac (IL2 recptor alpha subunit) with C-terminally tagged GFP in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

Tac (IL2RA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2261

Plasmid

#36700

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BMPR1A-TALEN_Right (BMPR1A Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

July 18, 2012

Availability

Academic Institutions and Nonprofits only
TAL2260

Plasmid

#36699

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BMPR1A-TALEN_Left (BMPR1A Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-C71,72,105A

Plasmid

#58396

Purpose

Expressed murine IFITM3-C71,72,105A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount, Howard Hang

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Cysteines 71, 72, and 105 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
GFP-Tac

Plasmid

#162489

Purpose

Expresses Tac (IL2 recptor alpha subunit) with GFP tag in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

Tac (IL2RA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-nTR

Plasmid

#21812

Depositor

Wafik El-Deiry

Article

Kim et al J Biol Chem. 2004 Sep 17. 279(38):40044-

Insert

pQE-nTR (TNFSF10 Human)

Use

Tags

Expression

Bacterial

Mutation

His tag and aa95-113 were removed from pQE-hTR

Promoter

Available since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA_DiCre_59.F2

Plasmid

#60207

Purpose

Expresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)

Depositor

Jean-Paul Herman

Article

Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

Insert

Cre recombinase (CD207 Bacteriophage P1, Human, Synthetic)

Use

Tags

FKBP12 and Nuclear localisation signal

Expression

Mammalian

Mutation

amino acids 19-59 of the humanized version of Cre…

Promoter

CMV

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
dTomato_LD 0_CS G_tTA-BFP

Plasmid

#58871

Purpose

MESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence

Use

AAV

Tags

EBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry_LD 0_CS G_tTA-BFP

Plasmid

#58856

Purpose

MESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence

Use

AAV

Tags

EBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
dTomato_LD 0_CS M_tTA-BFP

Plasmid

#58875

Purpose

MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')

Use

AAV

Tags

EBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM-mouse TCR zeta/2451

Plasmid

#11506

Depositor

Allan Weissman

Article

Weissman et al Science. 1988 Feb 26. 239(4843):1018-21.

Insert

TCR zeta (Cd247 Mouse)

Use

General cloning

Tags

Expression

Mutation

n/a

Promoter

Available since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K83,88,104A

Plasmid

#58405

Purpose

Expresses murine IFITM3-K83,88,104A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysines 83, 88, and 104 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K83A

Plasmid

#58401

Purpose

Expresses murine IFITM3-K83A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysine 83 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K24A

Plasmid

#58400

Purpose

Expresses murine IFITM3-K24A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysine 24 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
CD4_LD 0_CS G_tTA-BFP

Plasmid

#58869

Purpose

MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence

Use

AAV

Tags

EBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge