GFP-Tac
(Plasmid
#162489)
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PurposeExpresses Tac (IL2 recptor alpha subunit) with GFP tag in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 162489 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4770
- Total vector size (bp) 5523
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Modifications to backboneThe signal peptide of Tac was inserted before GFP tag
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTac
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Alt nameIL2 receptor alpha subunit
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SpeciesH. sapiens (human)
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Insert Size (bp)753
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GenBank IDNM_000417.3
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Entrez GeneIL2RA (a.k.a. CD25, IDDM10, IL2R, IMD41, TCGFR, p55)
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer EGFP-CF
- 3′ sequencing primer SV40-PArev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-Tac was a gift from Lei Lu (Addgene plasmid # 162489 ; http://n2t.net/addgene:162489 ; RRID:Addgene_162489) -
For your References section:
Glycans function as a Golgi export signal to promote the constitutive exocytic trafficking. Sun X, Tie HC, Chen B, Lu L. J Biol Chem. 2020 Aug 21. pii: RA120.014476. doi: 10.1074/jbc.RA120.014476. 10.1074/jbc.RA120.014476 PubMed 32826314