Tac-GFP
(Plasmid #162494)

• Purpose: Expresses Tac (IL2 recptor alpha subunit) with C-terminally tagged GFP in mammalian cells
• Depositing Lab: Lei Lu
• Publication: Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014476. doi: 10.1074/jbc.RA120.014476.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4701
• Total vector size (bp): 5523
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tac
• Alt name: IL2 receptor alpha subunit
• Species: H. sapiens (human)
• Insert Size (bp): 822
• GenBank ID: NM_000417.3
• Entrez Gene: IL2RA (a.k.a. CD25, IDDM10, IL2R, IMD41, TCGFR, p55)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-NR

Resource Information

• Supplemental Documents:
  • Tac-GFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry