-
Plasmid#168477PurposeC-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168475: pET11a-Z-N-DiB-RM to obtain DiB-RM‐split-Zip proteinDepositorInsertLeucine Zipper + C-fragment of DiB-RM
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-Z-N-DiB-RM
Plasmid#168475PurposeN-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168477: pMRBad-Z-C-DiB-RM to obtain DiB-RM‐split-Zip proteinDepositorInsertN-fragment of DiB-RM + Leucine Zipper
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ELK1_iso1
Plasmid#135737PurposeDonor vector for 3' FLAG tag of human ELK1_iso1DepositorInsertELK1_iso1 Homology arms (ELK1 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_MAF1_iso1
Plasmid#135732PurposeDonor vector for 3' FLAG tag of human MAF1_iso1DepositorInsertMAF1_iso1 Homology arms (MAF1 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX330_MAF1_iso1_2
Plasmid#135750PurposeEncodes gRNA for 3' target of human MAF1_iso1DepositorInsertMAF1_iso1 gRNA (MAF1 Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJan. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX330_ELK1_iso1_1
Plasmid#135757PurposeEncodes gRNA for 3' target of human ELK1_iso1DepositorInsertELK1_iso1 gRNA (ELK1 Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJan. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX330_ELK1_iso1_2
Plasmid#135758PurposeEncodes gRNA for 3' target of human ELK1_iso1DepositorInsertELK1_iso1 gRNA (ELK1 Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJan. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX330_E2F4_iso1_1
Plasmid#135756PurposeEncodes gRNA for 3' target of human E2F4_iso1DepositorInsertE2F4_iso1 gRNA (E2F4 Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJan. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX330_MAF1_iso1_1
Plasmid#135749PurposeEncodes gRNA for 3' target of human MAF1_iso1DepositorInsertMAF1_iso1 gRNA (MAF1 Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJan. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL1-neoR
Plasmid#117048PurposeIn vitro expression of neoR (kanamycin resistance gene) from the T7 promoterDepositorInsertneoR
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX458_HOMEZ_1
Plasmid#86352PurposeEncodes gRNA for 3' target of human HOMEZDepositorInsertgRNA against HOMEZ (HOMEZ Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF652_2
Plasmid#86317PurposeEncodes gRNA for 3' target of human ZNF652DepositorInsertgRNA against ZNF652 (ZNF652 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_HOMEZ
Plasmid#86289PurposeDonor vector for 3' FLAG tag of human HOMEZDepositorInsertHOMEZ homology arms (HOMEZ Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_HOMEZ_2
Plasmid#86353PurposeEncodes gRNA for 3' target of human HOMEZDepositorInsertgRNA against HOMEZ (HOMEZ Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_TFE3_1
Plasmid#86325PurposeEncodes gRNA for 3' target of human TFE3DepositorInsertgRNA against TFE3 (TFE3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_HBP1_1
Plasmid#86320PurposeEncodes gRNA for 3' target of human HBP1DepositorInsertgRNA against HBP1 (HBP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF652_1
Plasmid#86316PurposeEncodes gRNA for 3' target of human ZNF652DepositorInsertgRNA against ZNF652 (ZNF652 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_HBP1
Plasmid#86272PurposeDonor vector for 3' FLAG tag of human HBP1DepositorInsertHBP1 homology arms (HBP1 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHL391
Plasmid#62821PurposeLim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::RBS (st7) hfq::mCherry::T1 terminator + ColE1DepositorInsertsgfp
mCherry
UseSynthetic BiologyTagshfp leader, ompC leader, and st7 RBSExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL518
Plasmid#62823PurposeLim Lab plasmid: AmpR + PLlacO-1::micC::mCherry::T1 terminator + PLtetO-1m9::OmpC::gfp::T1T2 terminator + ColE1DepositorInsertsmCherry
gfp
UseSynthetic BiologyTagsm9::ompC leader and micC leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2021
Plasmid#62833PurposeLim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1DepositorInsertsT710 hfq leader
gfp
UseSynthetic BiologyTagsm9::sodB leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2026
Plasmid#62834PurposeLim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1DepositorInsertsT710 hfq leader
gfp
UseSynthetic BiologyTagsm9::glmS leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2027
Plasmid#62835PurposeLim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1DepositorInsertsT710 hfq leader
gfp
UseSynthetic BiologyTagsglmS leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2053
Plasmid#62836PurposeLim Lab plasmid: AmpR + PLlacO-1::T710::hfq::T1 termintor + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1DepositorInsertsT710 hfq leader
gfp
UseSynthetic BiologyTagsm9::ompC leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2073
Plasmid#62837PurposeLim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1DepositorInsertsglmZ leader
T710 hfq leader
gfp
UseSynthetic BiologyTagsm9::glmSExpressionBacterialMutationPromoterpConShortNoHind, pLlacO-1, and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2075
Plasmid#62838PurposeLim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1DepositorInsertsglmZ leader
T710 hfq leader
gfp
UseSynthetic BiologyTagsglmSExpressionBacterialMutationPromoterpConShortNoHind, pLlacO-1, and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2085
Plasmid#62840PurposeLim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1DepositorInsertsm8::glmZ leader
T710 hfq leader
gfp
UseSynthetic BiologyTagsglmSExpressionBacterialMutationPromoterpConShortNoHind, pLlacO-1, and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2117
Plasmid#62842PurposeLim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1DepositorInsertsM8::ryhB leader
gfp
UseSynthetic BiologyTagsm9::sodB leaderExpressionBacterialMutationPromoterpConShortNoHind and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2084
Plasmid#62839PurposeLim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1DepositorInsertsm8::glmZ leader
T710 hfq leader
gfp
UseSynthetic BiologyTagsm9::glmSExpressionBacterialMutationPromoterpConShortNoHind, pLlacO-1, and pLtetO-1Available sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVD419
Plasmid#63754Purpose3.4 kb HindIII fragment from EHEC associated plasmid from E. coli 0157:H7 strain 933 cloned into pBR325. Contains the hlyA gene. Best EHEC probe for detection of this plasmid.DepositorInsertehxA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-Map7-1783-end
Plasmid#46071DepositorInsertMap7-1783-end (Map7 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-Map7-856-1351
Plasmid#46069DepositorInsertMap7-856-1351 (Map7 Mouse)
UseUnspecifiedTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
hs-FL-Kek1-myc2
Plasmid#41170DepositorInsertKek1 (kek1 Fly)
UseTags2X mycExpressionInsectMutationPromoterHsp70Available sinceJan. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-mScarlet
Plasmid#131000PurposeAAV vector to drive the expression of mScarlet under the control of CamKIIa promoterDepositorHas ServiceAAV8InsertmScarlet
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLM-CMV-R-Cre
Plasmid#27546PurposeLentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.DepositorInsertmCherry_P2A_Cre recombinase
UseCre/Lox and LentiviralTagsExpressionMutationPromoterCMVAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
MSGV-1D3-28Z All ITAMs intact
Plasmid#107226PurposeAnti-mouse CD19 CAR with CD3 ITAMs intactDepositorInsert1D3-28Z All ITAMs intact
UseRetroviralTagsNoneExpressionMutationNonePromoterAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-NLS-mRuby2
Plasmid#99130PurposeAn AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancerDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV8, and AAV9InsertNLS-mRuby2
UseAAVTagsExpressionMammalianMutationPromotermDLX5/6Available sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MITF_P2A_Hygro_Barcode
Plasmid#120460PurposeBarcoded lentiviral vector to express MITF in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMITF (MITF Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
2xHP1bCD_W42A-IFP2.0C
Plasmid#215751PurposeExpresses binding-deficient W42A mutant of 2xHP1beta chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K9me2/3 detection in BiAD sensorsDepositorInsert2xHP1bCD_W42A-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG-BFP-sesRNA-GFP-WPRE-pA
Plasmid#192063PurposeExpression of BFP, sesRNA in mammalian cells, with GFP as efRNA.DepositorInsertsesRNA for tdTom
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only