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  1. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...for yes-no readouts.      Figure 4: A histogram plot for a smeary antibody readout. The orange...walk you through how to interpret the antibody readout on a flow plot.  Note that for the flow plots discussed...positive population between 103.5–104.5 for a cleaner readout. A yes-no plot If you are using an antibody to... a smeary dot plot showing a range of antibody readouts. (B) A gate on the smeary dot plot shows the population...of a histogram plot for a yes/no flow cytometry readout. Created with biorender.com.   Using histogram...

  2. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...their favorite submission. So without any further ado, we present to you: our first, second, and third ...

  5. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...session on our YouTube channel. And with no more ado, the questions!  AAV Prep and Technique Q1: Is there...found this to strongly bias titer. We are also adopting ddPCR, which does not a standard curve and somewhat...

  6. The Pleiades Promoter Project

    Type
    Collection
    ...intron-lacZ/NLS Ple2 ADORA2A pEMS1142 EGFP/NLS Ple3 ADORA2A pEMS1143 EGFP/NLS Ple3 ADORA2A pEMS1473 intron-...intron-lacZ/NLS Ple4 ADORA2A pEMS1144 EGFP/NLS Ple7 ATP6V1C2 pEMS1439 EGFP/NLS Ple9 ATP6V1C2 pEMS1078 EGFP/...

  7. Immunology Research Plasmids and Resources

    Type
    Collection
    ...TSHA CGB chorionic gonadotropin, beta polypeptide CGB3, hCGB CGB1 chorionic gonadotropin, beta polypeptide... 1 - CGB2 chorionic gonadotropin, beta polypeptide 2 - CGB5 chorionic gonadotropin, beta polypeptide 5...chorionic gonadotropin, beta polypeptide 7 CG-beta-a, FLJ35403, FLJ43118 CGB8 chorionic gonadotropin, beta...LHRH, LNRH GNRH2 gonadotropin-releasing hormone 2 GnRH-II, LH-RHII GNRHR gonadotropin-releasing hormone...maturation factor, gamma GMF-GAMMA, MGC126867 GNRH1 gonadotropin-releasing hormone 1 (luteinizing-releasing hormone...CGB4, LSH-B, hLHB LHCGR luteinizing hormone/choriogonadotropin receptor FLJ41504, LCGR, LGR2, LH/CG-R, LH...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... sensor GRAB_Ado1.0 in neurons or astrocytes, (Constitutive or Cre-dependent) Li Lab GRAB_Ado Plasmids...synapses. Neuron. 2006 Sep 21. 51(6):773-86. Leon Lagnado Endo/Exocytosis Synaptophysin with tandem repeats...Dinneny Lysosomal pH Lyso-pHluorin for fluorescent readout of lysosome acidification Optogenetic acidification...

  9. AAVED

    Type
    Collection
    ...We hope that this guide will improve technology adoption and scientific outcomes. Event Details Date: Friday...and you will be helping scientists successfully adopt these tools. Thank you for your contributions and...

  10. CRISPR Guide

    Type
    Collection
    ...Theunissen, T. W., Rangarajan, S., Shivalila, C. S., Dadon, D. B., & Jaenisch, R. (2013). Multiplexed activation...Communications , 9 (1). PMID: 30082838 Maddalo, D., Manchado, E., Concepcion, C. P., Bonetti, C., Vidigal, ..., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J.,...Partridge, E. C., Newberry, K. M., Smith, S. B., Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall...

  11. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...exchange vectors here! ShadowR, a novel FLIM-FRET acceptor Figure 4: ShadowR is used as a FRET acceptor...or mScarlet. Image: Murakoshi et al., 2019. ShadowR is an orange light-absorbing chromoprotein that...FLIM-FRET) in living cells (Figure 4). They created ShadowR by modifying the surface of the chromoprotein Ultramarine...binding to proteins can inhibit their activity. ShadowR can be used as a FRET acceptor in combination with...or mScarlet as the donor in HeLa cells.   Find ShadowR for mammalian cell expression here   Now that you...M., Sato, A., Koga, N., & Nabekura, J. (2019). ShadowR: a novel chromoprotein with reduced non-specific...

  12. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...lab has developed two new FLIM-FRET acceptors, ShadowG, a novel dark green fluorescent protein (GFP) with... with an absorption peak of 486 nm and ShadowY, a novel dark yellow fluorescent protein (YFP) with an ...fluorescence lifetime imaging microscopy) assays. ShadowG is darker than other dark YFP mutants, it reduces...desired due to its very low quantum efficiency. ShadowY has a lower quantum yield and higher absorption...reduced artifacts due to residual fluorescence. ShadowY was additionally shown to have a 7-fold lower quantum...

  14. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ... labs approach the situation differently, some adopting simple note-taking tools, others using excel sheets...team conducted a large study which focused on the adoption of ELNs in labs and included hundreds of researchers... In addition, we also explored the barriers to adoption within academic environments to better understand...indicated that cost was a significant barrier to ELN adoption. This includes financial outlay, staff hours, ...

  15. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...recognized and cleaved the target RNA sequence, they adopt an enzymatically active state and can bind and cleave...

  16. Plasmids for Optogenetics Research

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    Collection
    ...manner. One of the first microbial opsins to be adopted as an optogenetic tool is the widely used Channelrhodopsin...

  17. Jaenisch Lab CRISPR Plasmids

    Type
    Collection
    ...Katz Y, Theunissen TW, Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R. Cell Res. 2013 Oct;23(10):1163-71...

  18. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    ...access to professional-grade design software, like Adobe Illustrator, and I didn’t want to pay for these ...mouse. Vectr Vectr is like a pared down version of Adobe Illustrator. There’s both a web and a desktop version...quickly. Inkscape Inkscape is the most similar to Adobe Illustrator out of the three options for drawing...

  19. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...system. Basically, biosensors provide quantifiable readouts for levels of different chemicals, hormones, proteins...signal depends on the system and the receptor, but readouts could involve fluorescence, pH change, heat, etc...signal transducer which will quantitatively give a readout for whatever the bio-recognition event is (fluorescence...

  20. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...-FLAG-IG EIF2AK2 Flag LTR VWM disease Seiichi Oyadomari 101848 pTO_CHMP2B_LAP CHMP2B LAP CMV ALS Daniel...) RNF216 His, 3C protease cleavage site T7 Hypogonadotropic hypogonadism Bernhard Lechtenberg 171923 pOPINB-hRNF216...) RNF216 His, 3C protease cleavage site T7 Hypogonadotropic hypogonadism Bernhard Lechtenberg 172072 EIF2B3...Ward 178167 RNF216_Halo_C_allele RNF216 Halo Hypogonadotropic hypogonadism Michael Ward 178168 SERPINI1_...
Showing: 1 - 20 of 155 results