...for yes-no readouts.      Figure 4: A histogram plot for a smeary antibody readout. The orange...walk you through how to interpret the antibody readout on a flow plot.  Note that for the flow plots discussed...positive population between 103.5–104.5 for a cleaner readout. A yes-no plot If you are using an antibody to... a smeary dot plot showing a range of antibody readouts. (B) A gate on the smeary dot plot shows the population...of a histogram plot for a yes/no flow cytometry readout. Created with biorender.com.   Using histogram...

...their favorite submission. So without any further ado, we present to you: our first, second, and third ...

... genome editing experiments. So, without further ado, the most request plasmid in 2016 was... pSpCas9(...

...cloning experiment right away. And without further ado, here are the top ten! Top 10 blog posts from 2020...

...session on our YouTube channel. And with no more ado, the questions!  AAV Prep and Technique Q1: Is there...found this to strongly bias titer. We are also adopting ddPCR, which does not a standard curve and somewhat...

...exchange vectors here! ShadowR, a novel FLIM-FRET acceptor Figure 4: ShadowR is used as a FRET acceptor...or mScarlet. Image: Murakoshi et al., 2019. ShadowR is an orange light-absorbing chromoprotein that...FLIM-FRET) in living cells (Figure 4). They created ShadowR by modifying the surface of the chromoprotein Ultramarine...binding to proteins can inhibit their activity. ShadowR can be used as a FRET acceptor in combination with...or mScarlet as the donor in HeLa cells.   Find ShadowR for mammalian cell expression here   Now that you...M., Sato, A., Koga, N., & Nabekura, J. (2019). ShadowR: a novel chromoprotein with reduced non-specific...

...lab has developed two new FLIM-FRET acceptors, ShadowG, a novel dark green fluorescent protein (GFP) with... with an absorption peak of 486 nm and ShadowY, a novel dark yellow fluorescent protein (YFP) with an ...fluorescence lifetime imaging microscopy) assays. ShadowG is darker than other dark YFP mutants, it reduces...desired due to its very low quantum efficiency. ShadowY has a lower quantum yield and higher absorption...reduced artifacts due to residual fluorescence. ShadowY was additionally shown to have a 7-fold lower quantum...

...Biology at the University of Colorado Anschutz Medical Campus in Aurora, Colorado, USA. The primary focus ...Biology at the University of Colorado Anschutz Medical Campus in Aurora, Colorado, USA.     Richard Gronostajski...

... labs approach the situation differently, some adopting simple note-taking tools, others using excel sheets...team conducted a large study which focused on the adoption of ELNs in labs and included hundreds of researchers... In addition, we also explored the barriers to adoption within academic environments to better understand...indicated that cost was a significant barrier to ELN adoption. This includes financial outlay, staff hours, ...

...access to professional-grade design software, like Adobe Illustrator, and I didn’t want to pay for these ...mouse. Vectr Vectr is like a pared down version of Adobe Illustrator. There’s both a web and a desktop version...quickly. Inkscape Inkscape is the most similar to Adobe Illustrator out of the three options for drawing...

...system. Basically, biosensors provide quantifiable readouts for levels of different chemicals, hormones, proteins...signal depends on the system and the receptor, but readouts could involve fluorescence, pH change, heat, etc...signal transducer which will quantitatively give a readout for whatever the bio-recognition event is (fluorescence...

...talents and expertise Fortunately, the eLife ambassadors group was already thinking about ways to tackle... postdoc at Berkeley at the time and an eLife ambassador, had previous experience organizing open access...potentially enable reproducibility practices being adopted by a wider group of researchers. Generating material...

...consistently showing rapid development and quick adoption by the community. Our depositing labs continue... and use new tools. Because there’s such rapid adoption, we thought that requests would be biased towards...tools that fill needs in the community. The rapid adoption of new tools, illustrated in Figure 2, and the...

...marker of neuronal activation and is a sufficient readout to proceed with your behavioral experiments. Comparison... variable without the behavioral test. Another readout may be necessary to prove inhibition of neuronal...Monitoring behavioral output The appropriate behavioral readout for your optogenetics experiment is contained within...

...editor. Science, 384(6703), ado7082. doi: https://doi.org/10.1126/science.ado7082.  Nuñez, J. K., Chen, J...., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J.,...

...the cellular location of proteins, and can be a readout for protein interaction studies such as immunoprecipitation...and after treatment.  Westerns are also a useful readout for a number of cellular assays. They can determine...following immunoprecipitation and are a common readout for subcellular fractionations, a procedure that...

...is a PhD trained scientist at the University of Colorado, Anschutz Medical Campus.  He studies viruses ...currently a postdoctoral fellow at the University of Colorado Anschutz Medical Campus in Aurora, CO where she.... Erin is also the President of Project Bridge Colorado, an advocacy initiative that seeks to bridge the...

...Neurodegenerative and brain disorders are frequently overshadowed by other medical conditions, so these topics... The Addictive Brain, Dr. Sadangi is an eLife ambassador, SfN community leader, and an early research ...

...Benefits of the UBMTA: Peace of mind Addgene has adopted the terms of the Uniform Biological Material Transfer...by the National Institutes of Health (NIH) and adopted by the Association of University Technology Managers...

...science. “It ended up we moved to Colorado for my husband’s postdoc. Colorado is growing in the biotech sphere...