-
Plasmid#197095PurposeThis plasmid contains the coding sequence for signaling peptide M12, which contains the RPTAVEG signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M12, which contains the RPTAVEG signaling motif.
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M13_6xSAG
Plasmid#197096PurposeThis plasmid contains the coding sequence for the control peptide M13, which contains a linker with six repeats of serine-alanine-glycine. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CDepositorInsertControl peptide M13, which contains a linker with six repeats of serine-alanine-glycine (SAG)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTY186 EGFP-T2A-ALFA-ORF6 SARS-CoV-2
Plasmid#204976PurposeCo-express EGFP and SARS-CoV-2 ORF6 N-terminally labeled with ALFA tagDepositorInsertOpen Reading Frame 6 (ORF6 SARS-CoV-2)
UseTagsALFAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF702
Plasmid#209650PurposeSuicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicusDepositorInsertsDownstream homologous region
thermostable neomycin phosphotransferase gene
Upstream homologous region
UseTagsExpressionMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-human-pro-IL-18_V47N_I48N
Plasmid#214319PurposeExpression of gene in mammalian cells by retroviral transductionDepositorInsertIL18 (IL18 Human)
UseRetroviralTagsFlag and MycExpressionMutationV47N_I48NPromoterMESVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-human-pro-IL-18_LES33AAA
Plasmid#214321PurposeExpression of gene in mammalian cells by retroviral transductionDepositorInsertIL18 (IL18 Human)
UseRetroviralTagsFlag and MycExpressionMutationLES33AAAPromoterMESVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-human-pro-IL-18_DDEN29ENGD
Plasmid#214320PurposeExpression of gene in mammalian cells by retroviral transductionDepositorInsertIL18 (IL18 Human)
UseRetroviralTagsFlag and MycExpressionMutationDDEN29ENGDPromoterMESVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-human-pro-IL-18_E192K_D193K
Plasmid#214318PurposeExpression of gene in mammalian cells by retroviral transductionDepositorInsertIL18 (IL18 Human)
UseRetroviralTagsFlag and MycExpressionMutationE192K_D193KPromoterMESVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT_IFL_G1A-T2A_R
Plasmid#200183PurposeWT PA14 I-F Leader and G1A-T2A RepeatDepositorInsertWildtype I-F Leader and a mutant I-F Locus (G1A-T2A)
UseTagsExpressionBacterialMutationG1A-T2A in the I-F repeatPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW pHluorin-SYT9 IRES mRuby3
Plasmid#195698PurposeLentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoterDepositorInsertSyt9 (Syt5 Rat)
UseLentiviralTagspHluorinExpressionMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Syt9 IRES mRuby3
Plasmid#195700PurposeLentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoterDepositorInsertSyt9 (Syt5 Mouse)
UseLentiviralTagsExpressionMutationencodes only the first 68 amino acids of Tac1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2B D330, 332N
Plasmid#195705PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2B domain with D330, 332N mutationsDepositorInsertSyt9 (Syt5 Mouse)
UseTagsGSTExpressionBacterialMutationencodes amino acids 253-344 of mouse SYT9 with D3…PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2AB D197,199,330,332N
Plasmid#195706PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2AB domain with D197, 199, 330, 332N mutationsDepositorInsertSyt9 (Syt5 Mouse)
UseTagsGSTExpressionBacterialMutationencodes amino acids 104-386 of mouse SYT9 with D1…PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-HypaSpCas9-NLS-6xHis
Plasmid#207384PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertHypaSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationN692A, M694A, Q695A, H698APromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-evoSpCas9-NLS-6xHis
Plasmid#207389PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertevoSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationM495V, Y515N, K526E, R661QPromoterT7Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-eSpCas9-NLS-6xHis
Plasmid#207379PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationK848A, K1003A, R1060APromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYG215
Plasmid#200837PurposeTranscribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.DepositorInsertglmZ (glmZ Escherichia coli str. K-12 substr. MG1655)
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterP-LlacO-1Available sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEXPA10-NLS-3xmCitrine
Plasmid#192523PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertEXPANSIN A10 (EXPA10 Mustard Weed)
UseTags3xmCitrine and NLSExpressionPlantMutationPromoterEXPA10Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPIP1;4-NLS-3xmCitrine
Plasmid#192524PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertPLASMA MEMBRANE INTRINSIC PROTEIN 1;4 (PIP1;4 Mustard Weed)
UseTags3xmCitrine and NLSExpressionPlantMutationPromoterPIP1;4Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-mOrange2-CI
Plasmid#196852PurposeExpression of red fluorescent reporter mOrange driven by the strong Beta-actin promoterDepositorInsertmOrange2
UseTagsExpressionMammalianMutationNone (wt)PromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only