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Addgene

pCF702
(Plasmid #209650)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209650 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMTL80150
  • Backbone manufacturer
    https://plasmidvectors.com (Nigel Minton lab)
  • Backbone size w/o insert (bp) 2328
  • Total vector size (bp) 5653

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Downstream homologous region
  • Insert Size (bp)
    1010

Gene/Insert 2

  • Gene/Insert name
    thermostable neomycin phosphotransferase gene
  • Alt name
    NeoR
  • Insert Size (bp)
    1280

Gene/Insert 3

  • Gene/Insert name
    Upstream homologous region
  • Insert Size (bp)
    812

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pMTL80150: Heap JT, Pennington OJ, Cartman ST, Minton NP. 2009. A modular system
for Clostridium shuttle plasmids. J Microbiol Methods 78:79–85. https://
doi.org/10.1016/j.mimet.2009.05.004.

thermostable NeoR: pMU131 (Addgene #66811)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCF702 was a gift from Bastian Molitor (Addgene plasmid # 209650 ; http://n2t.net/addgene:209650 ; RRID:Addgene_209650)
  • For your References section:

    The Targeted Deletion of Genes Responsible for Expression of the Mth60 Fimbriae Leads to Loss of Cell-Cell Connections in Methanothermobacter thermautotrophicus DeltaH. Fink C, Martinez-Cano G, Shuster J, Panzera A, Rennhack KE, Rohbohm N, Angenent LT, Molitor B. Appl Environ Microbiol. 2023 Jul 26;89(7):e0057523. doi: 10.1128/aem.00575-23. Epub 2023 Jun 13. 10.1128/aem.00575-23 PubMed 37310347