pCF702
(Plasmid
#209650)
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PurposeSuicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209650 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMTL80150
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Backbone manufacturerhttps://plasmidvectors.com (Nigel Minton lab)
- Backbone size w/o insert (bp) 2328
- Total vector size (bp) 5653
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameDownstream homologous region
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Insert Size (bp)1010
Gene/Insert 2
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Gene/Insert namethermostable neomycin phosphotransferase gene
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Alt nameNeoR
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Insert Size (bp)1280
Gene/Insert 3
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Gene/Insert nameUpstream homologous region
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Insert Size (bp)812
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pMTL80150: Heap JT, Pennington OJ, Cartman ST, Minton NP. 2009. A modular system
for Clostridium shuttle plasmids. J Microbiol Methods 78:79–85. https://
doi.org/10.1016/j.mimet.2009.05.004.
thermostable NeoR: pMU131 (Addgene #66811)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCF702 was a gift from Bastian Molitor (Addgene plasmid # 209650 ; http://n2t.net/addgene:209650 ; RRID:Addgene_209650) -
For your References section:
The Targeted Deletion of Genes Responsible for Expression of the Mth60 Fimbriae Leads to Loss of Cell-Cell Connections in Methanothermobacter thermautotrophicus DeltaH. Fink C, Martinez-Cano G, Shuster J, Panzera A, Rennhack KE, Rohbohm N, Angenent LT, Molitor B. Appl Environ Microbiol. 2023 Jul 26;89(7):e0057523. doi: 10.1128/aem.00575-23. Epub 2023 Jun 13. 10.1128/aem.00575-23 PubMed 37310347