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Plasmid#79881PurposeUbiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
UseTagsAviExpressionBacterialMutationPromoterUbiquitin promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXL-CAG-ICeuI-Cerulean-2A-sHRPbNLG1
Plasmid#79912PurposepiggyBac transposon vector, Cerulean-2A-sHRPbNLG1DepositorInsertCerulean-2A-sHRPbNLG1
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2 Krt4 Src (YF)-uniRapR-cerulean
Plasmid#45509DepositorInsertSrc (YF)-uniRapR-cerulean
UseZebrafish expressionTagsceruleanExpressionMutationY732FPromoterkrt4Available sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
PhOTO-C (pMTB:cyto-Dendra2-2A-H2B-Cerulean)
Plasmid#92402PurposeConstitutive expression of cytosolic green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.DepositorInsertsDendra2
Cerulean
UseZebrafish expressionTagsH2B tag and No tag, cytosolic expressionExpressionMutationPromoterbactin2Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cerulean(159-238) in pcDNAI/Amp
Plasmid#60898PurposeThis is a vector that attaches Cerulean(159-238) to the N-terminus of a protein.DepositorInsertCerulean(159-238)
UseTagsExpressionMammalianMutationContains N164H substitutionPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSems leader-ALFA-mXFP-TpoR
Plasmid#222945PurposeExpression of TPOR with ALFAtag and monomeric XFP in mammalian cellsDepositorInsertThrombopoietin receptor (26-635) (MPL Human)
UseTagsALFAtag, Ig k-chain leader sequence, and mXFPExpressionMammalianMutationmXFP: tryptophan 66 to phenylalanine, TPOR: Signa…PromoterCMVAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME-mCerulean3-TUBB1A (JDW 1367)
Plasmid#224503PurposeGateway compatible middle entry clone containing mCerulean3 fused to TUBB1A for visualizing tubulin in cellsDepositorInsertmCerulean3-TUBB1A
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME H2B mCerulean (JDW 1150)
Plasmid#224543PurposeA Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleusDepositorInsertH2B-mCerulean
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 2A protease; no tag, self cleaving
Plasmid#204813PurposeBacterial expresison of Enterovirus A71 2A proteaseDepositorInsertA71 2A protease
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MsACR1-TS-Kv-P2A-mCerulean3-pAAV-CaMKII
Plasmid#204970PurposeSoma-targeted neuronal expression of MsACR1 and mCerulean3 separated by a P2A site.DepositorInsertMsACR1
UseAAVTagsmCerulean3ExpressionMutationPromoterCaMKIIAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MUC2 D1 with cleavable His tag
Plasmid#181813PurposeExpresses human MUC2 D1 with cleavable His tag, residues 21-389DepositorInsertMuc2 D1 (MUC2 Human)
UseTags6xHistadine followed by TEV cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC-DEST-cjSTELLA-mCerulean-PuroTK
Plasmid#141029PurposeReporter integrationDepositorInsertmCerulean
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
LOC5567066 Gastrin-releasing peptide receptor
Plasmid#115372PurposeExpresses Ae. aegypti receptor in mammalian cellsDepositorInsertLOC5567066 Gastrin-releasing peptide receptor (LOC5567066 Aedes aegypti)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cerulean(1-158)Stop in pcDNA1/amp
Plasmid#60894PurposeThis vector attaches mCerulean(1-158) to the C-terminus of a protein.DepositorInsertCerulean(1-158)Stop
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cerulean-Epac2(R53A)-Venus
Plasmid#138197PurposeFRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.DepositorInsertCerulean-Epac2(R53A)-Venus (Rapgef4 Mouse, Synthetic)
UseTagsCerulean and VenusExpressionMammalianMutationContains arginine-to-alanine substitution at resi…PromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cerulean-Epac2(R466A)-Venus
Plasmid#138199PurposeFRET-based Epac2 activation sensor with R466A mutation in Epac2 coding sequence.DepositorInsertCerulean-Epac2(R466A)-Venus (Rapgef4 Mouse, Synthetic)
UseTagsCerulean and VenusExpressionMammalianMutationContains arginine-to-alanine substitution at resi…PromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cerulean-Epac2(L426W)-Venus
Plasmid#138200PurposeFRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.DepositorInsertCerulean-Epac2(L426W)-Venus (Rapgef4 Mouse, Synthetic)
UseTagsCerulean and VenusExpressionMammalianMutationContains leucine-to-tryptophan substitution at re…PromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)
Plasmid#187844PurposeExpression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
UseTagsIg k-chain leader sequence, SNAPf-tag, and meGFPe…ExpressionMammalianMutationmeGFPe: asparagine 198 to aspartic acid and tyros…PromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry
Plasmid#34911DepositorInsertpresynaptic mGRASP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)
Plasmid#192785PurposeExpression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
UseTagsIg k-chain leader sequence, SNAPf-tag, and mXFPeExpressionMammalianMutationmXFPe: tyrosine 66 to phenylalanine, asparagine 1…PromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only