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Plasmid#128125PurposeExpressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032DepositorInsertsfGFP and BenR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBen and J23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E-EGFP-NS-MCS
Plasmid#75180PurposeEGFP (green) fluorophore with multiple cloning site for use with inducible N-terminal fusions.DepositorInsertEGFP-NS-MCS
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSW177 - pCASP1
Plasmid#115986Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpCASP1
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-FRB-HA no-pA
Plasmid#82594Purpose3' entry vector containing HA epitope fused to C-terminus of TOR FKBP and rapamycin binding (FRB) domain without pA for rapamycin/rapalog induced dimerizationDepositorInsertFRB-HA (no pA)
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-mos::NG
Plasmid#138958PurposeUsed to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4DepositorArticleInsertkkv (NEWENTRY Fly)
UseUas transgene vectorTagsNeon Green fused to C terminus of KkvExpressionInsectMutation8 bp that are in the region that encodes the cata…PromoterUASAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-004
Plasmid#78691PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-003
Plasmid#78690PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
C514-E23: attB5r-RU5u-3xFLAG-nostop-attB1r
Plasmid#162958PurposeGateway attB5r/attB1r entry clone for generation of N-terminal 3xFLAG-tagged fusion proteinsDepositorInserttriple FLAG epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
iTol2Amp-γ-crystallin:venusGFP
Plasmid#108462PurposeThis plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.DepositorInsertiTol2Amp crystGFP
UseTagsExpressionMutationPromoterAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
C234-E08: attB2r-3xFLAG-attB3
Plasmid#162912PurposeGateway attB2r/attB3 entry clone for construction of C-terminal triple-FLAG epitope tagged fusion proteinsDepositorInserttriple FLAG epitope tag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E44: attB5r-SNAP-nostop-attB1r
Plasmid#162962PurposeGateway attB5r/attB1r entry clone for generation of N-terminal SNAP-tagged fusion proteinsDepositorInsertSNAP tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r
Plasmid#162967PurposeGateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteinsDepositorInsertDual Strep2 purification tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
[GFP][IRES][Bla]
Plasmid#97194PurposeVector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.DepositorInsertsbeta-Lactamase
GFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-013
Plasmid#78701PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFP.R271
Plasmid#138220PurposeExpresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFTK086
Plasmid#171358PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertP-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-SGTAP no-pA
Plasmid#82599Purpose3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purificationDepositorInsertSBP-TEV-2x protein G
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3E-FRB(PLF)-HA no-pA
Plasmid#82597Purpose3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog induced dimerizationDepositorInsertFRB-HA (no pA)
UseTagsExpressionMutationK2095P, T2098L, W2101FPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5E-pax3a-5.4k
Plasmid#164966Purposezebrafish pax3a -5.4k promoterDepositorInsertpax3a (pax3a Zebrafish)
UseGateway 5 end entry vectorTagsExpressionMutationpromoter -5.4kPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW412hrpL-cIgfp
Plasmid#61438PurposepSB4A3 harbouring hrpL-rbs32-cI-lva-ter-Plam-rbs30-gfp-terDepositorInsertHrpL-rbs32-CI-LVA-t-plam-30gfp-t
UseTagsLVAExpressionBacterialMutationPromoterHrpLAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only