pFTK087
(Plasmid
#171359)
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PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171359 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepICH41331
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Vector typeBacterial Expression, Yeast Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameP-ANtRNA[Pro1]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
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gRNA/shRNA sequenceUnspecified
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SpeciesPenicillium, Aspergillus, or other fungal sources
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Mutationn/a
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFTK087 was a gift from Arnold Driessen (Addgene plasmid # 171359 ; http://n2t.net/addgene:171359 ; RRID:Addgene_171359) -
For your References section:
Modular Synthetic Biology Toolkit for Filamentous Fungi. Mozsik L, Pohl C, Meyer V, Bovenberg RAL, Nygard Y, Driessen AJM. ACS Synth Biol. 2021 Nov 2. doi: 10.1021/acssynbio.1c00260. 10.1021/acssynbio.1c00260 PubMed 34726388