pENTR1A_no_ccDB_mUty
(Plasmid #158570)

• Purpose: V5-tagged sequence of mUty cloned into entry vector for recombination
• Depositing Lab: Christian Deschepper
• Publication: Deschepper et al Sci Rep. 2020 Sep 10;10(1):14900. doi: 10.1038/s41598-020-71447-3.

Backbone

• Vector backbone: Addgene #17398
• Backbone size w/o insert (bp): 2294
• Total vector size (bp): 6116
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2
• Species: M. musculus (mouse)
• Insert Size (bp): 3822
• GenBank ID: NM_001326682.1
• Entrez Gene: Uty (a.k.a. Hydb, mKIAA4057)
• Tag / Fusion Protein:
  • V5 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: ApaI (destroyed during cloning)
• 5′ sequencing primer: pENTR-F CTACAAACTCTTCCTGTTAGTTAG
• 3′ sequencing primer: pENTR-R ATGGCTCATAACACCCCTTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.06.30.180232v1 for bioRxiv preprint.