-
Plasmid#132955PurposecrRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombeDepositorInsertdFnCas12a
UseCRISPR and Synthetic BiologyTagsSV40 NLS and nucleoplasmin NLSExpressionMutationPromoteradh1Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-mTagBFP-CAAX
Plasmid#75176PurposeMembrane targeted TagBFP (blue) fluorophore with stop codon for C-terminal fusionsDepositorInsertmTagBFP-CAAX
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-mOvol1
Plasmid#120359PurposeGateway entry vector containing mouse Ovol1.DepositorInsertOvol1 (Ovol1 Mouse)
UsePiggybac transposonTagsExpressionMammalianMutationPromotertet-on and CBhAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 TNRC6B
Plasmid#20022DepositorInsertTrinucleotide repeat containing 6B (TNRC6B Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-tRNA2.0
Plasmid#158396PurposeGolden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFP.R269
Plasmid#138181PurposeExpresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-YWHAE
Plasmid#31562DepositorInserttyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDrop
Plasmid#184872PurposeChromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFP.R272
Plasmid#138221PurposeExpresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-mCerulean3
Plasmid#75156PurposeNuclear mCerulean2 (blue) fluorophore for use with 5’ promoters.DepositorInsertH2B-mCerulean3
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJG80
Plasmid#89282PurposeEntry vector containing TaCas9-nos terminator between attL1 and attR5 sitesDepositorInsertTaCas9
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET19b+H2B(T116C)
Plasmid#157808PurposeExpression of human histone H2B with T116C mutation to facilitate maleimide chemistryDepositorInsertH2B(T116C)
UseTagsExpressionBacterialMutationT116CPromoterT7Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c
Plasmid#107599PurposeAn entry vector with U6a and U6c promoter driving guide RNAs expressionDepositorInsertsU6a promoter
U6c promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M2-TLR2
Plasmid#12291DepositorInsertTLR2 (Tlr2 Mouse)
UseTagsFLAG (FLAG-M2)ExpressionMammalianMutationM82I and H202Y; signal peptide (aa1-23) replaced …PromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Cas12j2
Plasmid#173926PurposeGateway entry clone (attL5 & attL2) for Cas12j2 crRNA expression under ZmUbi promoter with ribozyme processingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135-tRNA2.0
Plasmid#158397PurposeGolden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR4_FUS
Plasmid#26366DepositorInsertFUS (FUS Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceSept. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pME-Gal80ts
Plasmid#51619Purposevertebrate expression of full-length temperature-sensitive Gal80 with stopDepositorInsertGal80ts (GAL80 Budding Yeast)
UseVertebrate expressionTagsExpressionMutationtemperature sensitive (G203V, G323R)*PromoterAvailable sinceMarch 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR2B-dCas9-KRAB
Plasmid#115547PurposeEntry cassette for dCas9-KRABDepositorInsertdCas9-KRAB
UseTagsHAExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFP.R275
Plasmid#138224PurposeExpresses the split mCherry reporter interrupted by the PhoRadA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only