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Spatial and temporal control of transgene expression in zebrafish.

Akerberg AA, Stewart S, Stankunas K
PLoS One. 2014 Mar 18;9(3):e92217. doi: 10.1371/journal.pone.0092217. eCollection 2014. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
82582p5E-EF1a/b-globin5' entry vector containing frog translation elongation factor 1 alpha (EF1a) enhancer fused to rabbit b-globin intron for semi-ubiquitous expression
82584p5E-dusp65' entry vector containing FGF-responsive regulatory element of the zebrafish dusp6 promoter for FGF-responding cells
82585p5E-krt55' entry vector containing the zebrafish krt5 promoter for epidermis-specific expression
82587pME-ERT2-Cre-ERT2Middle entry vector containing tamoxifen-inducible estrogen receptor (ERT2) fused to both ends of CRE recombinase for tamoxifen-inducible Cre recombination
82588pME-GAL4-ERT2-VP16Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

Antibodies from Article