Spatial and temporal control of transgene expression in zebrafish.
Akerberg AA, Stewart S, Stankunas K
PLoS One. 2014 Mar 18;9(3):e92217. doi: 10.1371/journal.pone.0092217. eCollection 2014.
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PubMed
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
82582 | p5E-EF1a/b-globin | 5' entry vector containing frog translation elongation factor 1 alpha (EF1a) enhancer fused to rabbit b-globin intron for semi-ubiquitous expression |
82584 | p5E-dusp6 | 5' entry vector containing FGF-responsive regulatory element of the zebrafish dusp6 promoter for FGF-responding cells |
82585 | p5E-krt5 | 5' entry vector containing the zebrafish krt5 promoter for epidermis-specific expression |
82587 | pME-ERT2-Cre-ERT2 | Middle entry vector containing tamoxifen-inducible estrogen receptor (ERT2) fused to both ends of CRE recombinase for tamoxifen-inducible Cre recombination |
82588 | pME-GAL4-ERT2-VP16 | Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression |