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4xHRE_RE:RedF

Plasmid

#178314

Purpose

HRE RedFirefly luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

HRE RedFirefly reporter

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET28CLY3

Plasmid

#21762

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 3 SGGSGG repeats

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pET28_mouse-Caspase-1_enzymatic-domain

Plasmid

#183385

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1 (AA130-404) (Casp1 Mouse)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pNHEJ-Cas12a

Plasmid

#158713

Purpose

CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinum

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

the MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_MYC_WT

Plasmid

#82927

Purpose

Gateway Donor vector containing MYC, part of the Target Accelerator Plasmid Collection.

Depositor

Matthew Meyerson, Jesse Boehm, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

Myc (MYC Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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JB84

Plasmid

#128908

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab9AQL-BirA*-HA-MAO (RAB9A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-CD81-10

Plasmid

#55012

Purpose

Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

CD81 (CD81 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-PACT

Plasmid

#50352

Purpose

bacterial expression of PACT

Depositor

Kumiko Ui-Tei

Article

Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

Insert

PACT (PRKRA Human)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-Rab5CA(Q79L)

Plasmid

#35138

Depositor

Sergio Grinstein

Article

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Insert

Rab5a (RAB5A Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Constitutively Active- Q79L

Promoter

CMV

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

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PB-PG13-mCherry-EF1a-PuroR-EGFP

Plasmid

#195292

Purpose

piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

Depositor

Hideyuki Oguro

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

mCherry
PuroR-P2A-EGFP

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ccdB-3xFLAG-V5

Plasmid

#87063

Purpose

Mammalian Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

Type

Empty backbone

Use

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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Tet-inducible mCherry reporter

Plasmid

#64128

Purpose

Photoactivatable transcription system. mCherry reporter under the control of a tet-inducible promoter

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetOx13-CMV

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

Plasmid

#183951

Purpose

Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Insert

APOBEC1 (Apobec1 Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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N-Terminal Split Cas9 with GyrA intein

Plasmid

#58693

Purpose

Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

Depositor

Gang Bao

Article

Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

Insert

Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein

Use

AAV and CRISPR

Tags

3xFlag, GyrA Nsplit Intein, and NLS

Expression

Mammalian

Mutation

Promoter

CBh

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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PB-iCas9

Plasmid

#160048

Purpose

inducible expression of Cas9

Depositor

Xiaojun Lian

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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R703-M72-303: CMV51p> Halotag-Hs.KRAS4b

Plasmid

#159679

Purpose

Mammalian protein expression of KRAS4b-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Hs.KRAS4b (KRAS Human)

Use

Tags

Halotag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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JB85

Plasmid

#128909

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab9ASN-BirA*-HA-MAO (RAB9A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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PiggyBac-TetO-Ascl1-neomycin

Plasmid

#176482

Purpose

TetO-Ascl1 in a PiggyBac vector with neomycin resistance

Depositor

Marius Wernig

Article

Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

Insert

Ascl1 (Ascl1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pIRE YIC

Plasmid

#18784

Depositor

Han Htun

Article

Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

Insert

H-ferritin iron response element

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-Q23

Plasmid

#40261

Depositor

David Rubinsztein

Article

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Insert

HTT partial exon 1 Q23 (HTT Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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