PB-PG13-mCherry-EF1a-PuroR-EGFP
(Plasmid #195292)

• Purpose: piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)
• Depositing Lab: Hideyuki Oguro
• Publication: Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1702.e22. doi: 10.1016/j.stem.2022.11.004.

Backbone

• Vector backbone: PB-EF1a-PuroR-EGFP
• Backbone manufacturer: H. Oguro, unpublished
• Total vector size (bp): 11238
• Vector type: Mammalian Expression ; piggyBac transposon
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: mCherry
• Species: Synthetic
• Insert Size (bp): 711

Gene/Insert 2

• Gene/Insert name: PuroR-P2A-EGFP
• Alt name: Puromycin-N-acetyltransferase
• Alt name: Enhanced green fluorescent protein
• Species: Synthetic
• Insert Size (bp): 1389

Resource Information

• Supplemental Documents:
  • PB-PG13-mCherry-EF1a-PuroR-EGFP.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was generated by inserting the PG13-mCherry sequence (Addgene 195291) into the PB-EF1a-PuroR-EGFP plasmid (H. Oguro, unpublished), and contains the piggyBac 3'-inverted terminal repeat (PB 3'ITR), 5'-HS4 chicken b-globin (cHS4) insulator, PG13-mCherry reporter, woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), bovine growth hormone polyadenylation signal (bGHpA), EF1a promoter, puromycin registrant gene, P2A peptide, EGFP, bGHpA, cHS4 insulator, and PB 5'ITR, in this order.