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Plasmid#20255DepositorInsertUbiquitin-20 amino acid extension (RBX1 Human)
UseTagsmycExpressionMammalianMutationUbiquitin fused to N-terminus of GFP. Glycine 76 …PromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRmHa3-dKDM4A-H195A-HA2FL2
Plasmid#20235DepositorInsertdKDM4A (Kdm4A Fly)
UseTagsFLAG2 and HA2ExpressionInsectMutationchanged His 195 to AlaPromoterAvailable sinceMarch 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
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Neurl1-TOPO
Plasmid#17281DepositorInsertNeuralized-like (Neurl1a Mouse)
UseTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
M29 NacMS+LEF>Nac
Plasmid#17179DepositorInsertnacre promoter driving nacre cDNA (mitfa Zebrafish)
UseTagsExpressionMutationAll three Tcf/Lef sites are mutated, but 5 LEF si…PromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK E7 del EDE
Plasmid#13675DepositorInsertHPV 16 E7 del EDE (E7 Human)
UseTagsGSTExpressionBacterialMutationdel aa 35-37PromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
RCAS (A) Radical Fringe (CT#475)
Plasmid#13981DepositorInsertRadical Fringe (RFNG Chicken)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
RCAS (B) Radical Fringe (CT#476)
Plasmid#13982DepositorInsertRadical Fringe (RFNG Chicken)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
RCAS (B) flag Radical Fringe (CT#472)
Plasmid#13983DepositorInsertRadical Fringe (RFNG Chicken)
UseRetroviral; Avian expressionTagsFlagExpressionMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
p031 pCS2 End-1 ORF
Plasmid#11030DepositorInsertEnd-1 (end-1 Nematode)
UseXenopus, mammalian, avian, and zebrafishTagsExpressionMutationPromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMT Draper-Ala11-GFP
Plasmid#126654PurposeInducible expression of Draper-Ala11-GFP mutant in S2 cellsDepositorInsertDraper cytoplasmi Tyr residues mutated to Ala
UseTagsExpressionInsectMutationCytoplasmic Tyr residues to AlaPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMT Draper-delta ITAM-GFP
Plasmid#126655PurposeInducible expression of Draper-delta ITAM-GFP mutant in S2 cellsDepositorInsertDraper with ITAM Tyr residues mutated to Ala
UseTagsExpressionInsectMutationCytoplasmic ITAM Tyr residues mutated to PhePromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMT FRB-Draper INT-GFP
Plasmid#126665PurposeInducible expression of a synthetic FRB-Draper receptor in S2 cellsDepositorInsertFRB-Draper INT domain
UseTagsExpressionInsectMutationK986N- see depositor comments belowPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET His10-Draper delta ITAM
Plasmid#126671PurposeExpression of His10-Draper delta ITAM mutant for purification from E. coliDepositorInsertDraper cytoplasmic ITAM residues mutated to Phe
UseTagsExpressionBacterialMutationCytoplasmic ITAM residues mutated to PhePromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLenti_dCas9-KRAB-MeCP2_Blast
Plasmid#221501PurposeLentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vectorDepositorInsertdCas9-KRAB-MeCP2-T2A-Blast
UseCRISPR and LentiviralTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
Luciferase-pcDNA3
Plasmid#18964DepositorInsertFirefly Luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
3X ERE TATA luc
Plasmid#11354PurposeLuciferase reporter containing three copies of vitellogenin Estrogen Response ElementDepositorInsertERE
UseLuciferaseTagsExpressionBacterialMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTara:500
Plasmid#60717PurposeExpresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORFDepositorInsertT7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a_TIA1
Plasmid#106095Purposebacterial protein expression of TIA1DepositorInsertTIA1 (TIA1 Human)
UseTags6xHIs-thrombin and T7ExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFAP-Cre
Plasmid#24704DepositorInsertCre
UseTagsExpressionMammalianMutationPromoterGFAP (glial fibrillary acidic protein)Available sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only