Showing: 5381 - 5400 of 16015 results
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Plasmid#58334PurposeExpresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged proteinDepositorInsertPepTso
UseTagsGFP-HisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pJL1-MBP-4xDQNAT
Plasmid#128390PurposeIn vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sitesDepositorInsertMaltose binding protein
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationA302T- please see depositor commentPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pJL1-TTlight-4xDQNAT
Plasmid#128394PurposeIn vitro expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sitesDepositorInsertTetanus toxin light chain domain
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationInactivating E234A mutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
YFP-FKBPx5-His
Plasmid#103785Purposeexpresses His-tagged iPOLYMER component in E. coli for protein purificationDepositorInsertYFP-FKBPx5-His
UseTagsEYFP, His-tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-hMCP-mScarlet-I-NLS
Plasmid#174878PurposePiggyBac transposon vector for expression of MCP-mScarlet-I-NLSDepositorInsertMCP-mScarlet-I-NLS
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28a(TIGIT)
Plasmid#177902PurposeBacterial expression of his10-tagged human TIGIT Intracellular DomainDepositorInsertTIGIT (aa 163-244 only) (TIGIT Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
GFP-GLT1a-V5
Plasmid#162517PurposeRat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loopDepositorInsertGLT1a (Slc1a2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
GFP-GLT1b-V5
Plasmid#162518PurposeRat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loopDepositorInsertGLT1b (Slc1a2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDUAL-P41nmt1-Nup124-GFP-MTn
Plasmid#154365PurposeYeast expression of Nup124DepositorInsertNup124 (nup124 Fission Yeast)
UseTagsExpressionYeastMutationSee depositor comments belowPromoterAvailabilityAcademic Institutions and Nonprofits only -
csd1_125-312/pET28b(+)
Plasmid#83290Purposecsd1, construct: 125-312, pET28b(+) NC-tagDepositorInserthp1543
UseTagsHis tagExpressionBacterialMutationdeleted amino acid 1-124PromoterAvailabilityAcademic Institutions and Nonprofits only -
csd2_121-308/pET28b(+)
Plasmid#83292Purposecsd2, construct: 121-308, pET28b(+) NC-tagDepositorInserthp1544
UseTagsHis tagExpressionBacterialMutationdeleted amino acid 1-120PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hOCT3/4-shp53-F + mCherry-2A-puro
Plasmid#74947PurposeDosage control and tracing of reprogramming episomesDepositorInsertOct3/4 (POU5F1 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MSCV-PIG-N3FLAG-TAF10
Plasmid#105990Purposeretrovirally express mouse TAF10 with 3*FLAG tag at N terminal, GFP marker and puro resistanceDepositorInsertFLAG tagged TAF10 (Taf10 Mouse)
UseRetroviralTags3*FLAGExpressionMammalianMutationPromoterMSCV-LTRAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)
Plasmid#115789PurposeExpresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.DepositorInsertNDP (NDP Human)
UseTagsalkaline phosphatase and flagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-REDD1-HA
Plasmid#133550PurposeExpresses HA tagged REDD1 in Mammalian cellsDepositorInsertRegulated in development and DNA damage response 1 (REDD1) (DDIT4 Human)
UseRetroviralTagsHA tagExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAG32-3HA-3FLAG
Plasmid#154081PurposeFor C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.DepositorInsert3xHA-3xFLAG-hygR
UsePcr-based tagging in yeastTags3xHA-3xFLAGExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPAICS-2XStrep
Plasmid#125683Purposeexpresses human PAICS protein with a C-terminal 2XStrep affinity tagDepositorInsertPAICS (PAICS Human)
UseTags2X Strep-Tag IIExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)
Plasmid#139445PurposeLentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoterDepositorInsertFLAG-tag E2-crimson
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.CMV/Flag-E2-crimson.IRES-puro.WPRE (MT06)
Plasmid#139448PurposeLentiviral vector to ectopically express Flag-tagged E2-crimson from CMV promoterDepositorInsertFLAG-tag E2-crimson
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pWMD23
Plasmid#140006PurposeExpression of ORF1 (Shuffle 1 NLS) and Pong TPase (L418A, L420A)DepositorInsertsORF1 Shuffle 1 NLS
Pong TPase LA
UseTagsExpressionPlantMutationNES sequence mutated L418A, L420A and Ping N-term…PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 5381 - 5400 of 16015 results