csd2_121-308/pET28b(+)
(Plasmid
#83292)
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Purposecsd2, construct: 121-308, pET28b(+) NC-tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 83292 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28b(+)
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehp1544
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Alt nameToxR-activated gene (TagE)
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SpeciesH. pylori 26695 strain
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Insert Size (bp)564
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Mutationdeleted amino acid 1-120
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GenBank IDAAD08583.1
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Tags
/ Fusion Proteins
- His tag (N terminal on backbone)
- His tag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nde1 (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
csd2_121-308/pET28b(+) was a gift from SeWon Suh (Addgene plasmid # 83292 ; http://n2t.net/addgene:83292 ; RRID:Addgene_83292) -
For your References section:
Structural Basis of the Heterodimer Formation between Cell Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori. An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, Mobashery S, Kim SJ, Suh SW. PLoS One. 2016 Oct 6;11(10):e0164243. doi: 10.1371/journal.pone.0164243. eCollection 2016. 10.1371/journal.pone.0164243 PubMed 27711177