Addgene

We narrowed to 636 results for: Bacillus

Showing: 521 - 540 of 636 results

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pCMV-AsCas12f1-v5.1+T1-empty

Plasmid

#204977

Purpose

AsCas12f1-v5.1+T1-based Human cell genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nature Catalysis (2023) 6, 695–709

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

N70Q/K103R/A104R/S118A/D364R

Promoter

PCMV,U6

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSL4164 (pEffector_IscB,ωRNA)

Plasmid

#210874

Purpose

Expresses GstIscB and native ωRNA from constitutive promoters

Depositor

Samuel H. Sternberg

Article

Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

Insert

GstIscB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-ermGFP

Plasmid

#27172

Depositor

Michela Lizier

Article

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pSL4672 (pEffector_TnpB3,ωRNA)

Plasmid

#210872

Purpose

Expresses GstTnpB3 and native ωRNA from constitutive promoters

Depositor

Samuel H. Sternberg

Article

Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

Insert

GstTnpB3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pTec_mCherry

Plasmid

#229145

Purpose

Promoter strength evaluation of Ptec

Depositor

Shrikrishnan Sankaran

Article

Blanch-Asensio et al Microb Cell Fact. 2024 Feb 7;23(1):42. d

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

No

Promoter

Ptec

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pSL4673 (pEffector_TnpB4,ωRNA)

Plasmid

#210873

Purpose

Expresses GstTnpB4 and native ωRNA from constitutive promoters

Depositor

Samuel H. Sternberg

Article

Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

Insert

GstTnpB4

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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M4KHX7

Plasmid

#163210

Purpose

Inducible expression of UniProt identifier M4KHX7

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

M4KHX7

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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B1HZY7

Plasmid

#163229

Purpose

Inducible expression of UniProt identifier B1HZY7

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

B1HZY7

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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I7IZ16

Plasmid

#163243

Purpose

Inducible expression of UniProt identifier I7IZ16

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

I7IZ16

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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G6F8W6

Plasmid

#163204

Purpose

Inducible expression of UniProt identifier G6F8W6

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

G6F8W6

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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Q5FIR1

Plasmid

#163287

Purpose

Inducible expression of UniProt identifier Q5FIR1

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

Q5FIR1

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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C4VMW0

Plasmid

#163249

Purpose

Inducible expression of UniProt identifier C4VMW0

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

C4VMW0

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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C0XIJ3

Plasmid

#163227

Purpose

Inducible expression of UniProt identifier C0XIJ3

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

C0XIJ3

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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C2K1F0

Plasmid

#163272

Purpose

Inducible expression of UniProt identifier C2K1F0

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

C2K1F0

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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F9UTP6

Plasmid

#163277

Purpose

Inducible expression of UniProt identifier F9UTP6

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

F9UTP6

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR-PsppA-mCherry

Plasmid

#225428

Purpose

Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

Depositor

Geir Mathiesen

Article

Wiull et al Applied and Industrial Microbiology 2025

Insert

mCherry, Cas9, SgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PsppA upstream of mCherry and Cas9, P3 upstream o…

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTRKH3-ermGFP

Plasmid

#27169

Depositor

Michela Lizier

Article

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5

Use

Tags

Expression

Bacterial

Mutation

presence of an additional 183-bp region at N term…

Promoter

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pLP3

Plasmid

#209958

Purpose

Entry vector to store Level 0 broad-host range L. plantarum replication origin parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pKAR2-Br512

Plasmid

#161875

Purpose

Expresses Br512 polymerase in E.coli

Depositor

Andrew Ellington

Article

Maranhao et al medRxiv

Insert

Modified DNA Polymerase I Large Fragment (Br512)

Use

Tags

8x His tag; Villin Headpiece 47 amino acid (HP47)…

Expression

Bacterial

Mutation

Promoter

T7, tetPA

Available since

Nov. 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pIntegrase_7

Plasmid

#60578

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_7

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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