-
-
Easing the Protein Purification Process with pCri
TypeBlog Post... Protein Tags. Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes...other than E. coli, specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...bacterial integrative and conjugative elements in Bacillus subtilis (ICEBs1) system. ICEBs1 carries the genes...ICEBs1, an integrative and conjugative element in Bacillus subtilis." Plasmid 86 (2016): 14-25. PubMed PMID... -
Synthetic Biology - Overview
TypeCollection...Multiplexed/Programmable Gene Networks Mascher Lab Bacillus BioBrick Box Mukhopadhyay Lab Efflux Pumps Voigt... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...Yersinia pestis, Agrobacterium tumefaciens, and Bacillus anthracis (Lui et al., 2012, Ni et al., 2008, ...Zhu L, Wang H (2012) Curing of Plasmid pXO1 from Bacillus anthracis Using Plasmid Incompatibility. PLoS ... -
What's New in CRISPR - Spring 2019
TypeBlog Post...cells. The Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that is functional at 37 °C...editing The best studied Cas12b nuclease from Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal... -
Microbiology Resources
TypeCollection...islandicus Plasmids for Bacteria Species Anabaena sp. Bacillus sp. Burkholderia sp. Bordetella pertussis Campylobacter...sp. Deinococcus radiodurans Enterococcus sp. Geobacillus sp. Haemophilus influenzae Helicobacter pylori... -
Plasmids 101: Repressible Promoters
TypeBlog Post... of other organisms, such as sphingomonads and bacillus. ADH1 The ADH1 negative repressible promoter ...synthetic cumate-inducible gene expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog Post...gene. sacB comes from the gram-positive bacteria Bacillus subtilis and encodes the enzyme levansucrase that... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog Post...involves cloning cry genes from the soil bacterium Bacillus thuringiensis and analysing the insecticidal potential... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...kit can be used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your... -
15 Hot Plasmids from 2017
TypeBlog Post...libraries The Gross Lab at UCSF recently deposited 2 Bacillus subtilis libraries with Addgene – the B. subtilis... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog Post... spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas campestris IncQ RSF1010...Gram-positive Acinetobacter calcoaceticus, Actinobacillus pleuropneumoniae, Actinomyces naeslundii, A..., Streptomyces lividans, Synechococcus spp., Thiobacillus ferrooxidans, Vibrio salmonicida, Yersinia enterocolitica... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog Post... Cas9 protein from the thermophilic bacteria Geobacillus stearothermophilus (GeoCas9). G. stearothermophilus... -
What's New in CRISPR - November 2021
TypeBlog Post...utilizes a Cas12f from a different species, Acidibacillus sulfuroxidans, AsCas12f1. They found that this... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post... research has shown that a Cas9 protein from Geobacillus stearothermophilus is more stable in human plasma...