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  2. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ... Protein Tags. Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes...other than E. coli, specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved...

  3. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...bacterial integrative and conjugative elements in Bacillus subtilis (ICEBs1) system. ICEBs1 carries the genes...ICEBs1, an integrative and conjugative element in Bacillus subtilis." Plasmid 86 (2016): 14-25. PubMed PMID...

  4. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...Yersinia pestis, Agrobacterium tumefaciens, and Bacillus anthracis (Lui et al., 2012, Ni et al., 2008, ...Zhu L, Wang H (2012) Curing of Plasmid pXO1 from Bacillus anthracis Using Plasmid Incompatibility. PLoS ...

  5. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...cells. The Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that is functional at 37 °C...editing The best studied Cas12b nuclease from Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal...

  6. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... of other organisms, such as sphingomonads and bacillus.  ADH1 The ADH1 negative repressible promoter ...synthetic cumate-inducible gene expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303...

  10. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...libraries The Gross Lab at UCSF recently deposited 2 Bacillus subtilis libraries with Addgene – the B. subtilis...

  11. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ... spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas campestris IncQ RSF1010...Gram-positive  Acinetobacter calcoaceticus, Actinobacillus pleuropneumoniae, Actinomyces naeslundii, A..., Streptomyces lividans, Synechococcus spp., Thiobacillus ferrooxidans, Vibrio salmonicida, Yersinia enterocolitica...

  12. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ... Cas9 protein from the thermophilic bacteria Geobacillus stearothermophilus (GeoCas9). G. stearothermophilus...

  13. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...utilizes a Cas12f from a different species, Acidibacillus sulfuroxidans, AsCas12f1. They found that this...
Showing: 1 - 14 of 14 results