-
Plasmid#171800PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB-EF1a-NLuc-IRES-Puro
Plasmid#130936PurposePiggyBac vector for constitutive NanoLuc expression in mammalian cellsDepositorInsertNanoLuc
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-GFP-progerin
Plasmid#17663DepositorInsertGFP-D50 lamin A (LMNA Human)
UseRetroviralTagsEGFPExpressionMammalianMutationDelta 50PromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Flag-cMyc T58A
Plasmid#20076DepositorInsertcMyc (MYC Human)
UseRetroviralTagsFlagExpressionMammalianMutationT58APromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pL6-H2B-iRFP720-puro
Plasmid#189992PurposeLentivirial plasmid for nuclear iRFP-720 expression with puromycin resistanceDepositorInsertHuman histone H2B
UseLentiviralTagsiRFP720ExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-pegRNA-puro backbone
Plasmid#214085PurposeLentiviral backbone plasmid to insert pegRNAs of interest. The cloning site is BsmbI.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterhU6, EF1aAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-myr-HA-Akt1
Plasmid#46969Purposelentiviral expression of myr-HA-Akt1DepositorInsertAKT1 (AKT1 Human)
UseLentiviralTagsHA and myrExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST PKAKTRClover
Plasmid#59153PurposeLentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertPKA (PRKACA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-P2A-EGFP_mRFPstuf
Plasmid#137730PurposeExpresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNADepositorTypeEmpty backboneUseCRISPR, Lentiviral, Mouse Targeting, and Syntheti…TagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP
Plasmid#67991PurposeCRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPREDepositorInsertU6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUW-Luc-mCherry-puro
Plasmid#183685PurposeLentiviral vector expressing luciferase and mCherry.DepositorInsertLuc-E2A-mCherry-T2A-PuroR
UseLentiviralTagsExpressionMutationPromoterHuman Ubiquitin C (UbC) promoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1
Plasmid#114022PurposeInducible expression of eGFP-tagged HEC1 in FLPin Trex cell linesDepositorInserteGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-ABERA-P2A-Puro
Plasmid#112675PurposeLentivirus for constitutive expression of ABE7.10 RA (codon optimized)DepositorInsertABERA
UseLentiviralTagsFLAGExpressionMutationD10APromoterEF1sAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
FU-H2B-GFP-IRES-Puro
Plasmid#69550PurposeLentiviral, selectable fluorescent GFP tagging of nuclei.DepositorInsertHistone 2B - green fluorescent protein fusion
UseLentiviralTagsGFP fusionExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
CA-RIT-NFAT1 (PGK-Puro)
Plasmid#63214PurposeConstitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interactio. Contains Puromycin selection markerDepositorInsertCA-RIT-NFAT1 (Nfatc2 Mouse)
UseRetroviralTagsHAExpressionMammalianMutationAmino acids 1-3 removed; CA: PASSGSSASF mutated t…PromoterAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-BRAF-V600E
Plasmid#15269PurposeRetroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)DepositorInsertBRAF (BRAF Human)
UseRetroviralTagsHis and MycExpressionMammalianMutationValine 600 to Glutamate (mutation has been found …PromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro-RFP-ATG9A
Plasmid#60609PurposeExpresses RFP-hATG9A in mammalian cellsDepositorInsertHomo sapiens autophagy related 9A (PPARG Human)
UseRetroviralTagsRFPExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PCNA-IRES-puro2b
Plasmid#26461DepositorInsertPCNA (PCNA Human)
UseTagspEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-Puro-2A-cGAS
Plasmid#102612PurposeLentivector expressing cGASDepositorInsertcGAS (CGAS Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF1a-ABCD3-RFP-Puro
Plasmid#170168PurposeMammalian Expression of Ef1a-ABCD3-RFP-PuroDepositorInsertABCD3-RFP-Puro
UseTagsExpressionMammalianMutationn/aPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)
Plasmid#176932PurposeDonor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locusDepositorInserthGEM(1-110)_mNG_hSLBP(18-108)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterPGK1Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLC-ZsGreen-P2A-Puro
Plasmid#124302PurposeLentiviral vector for expression of ZsGreen-P2A-Puro casette from a CMV promoterDepositorInsertZsGreen
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W
Plasmid#67977PurposeCRISPR gRNA expression vector with an improved scaffold and puro/mCherry markersDepositorInsertU6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro shKRAS
Plasmid#116871PurposeTet-inducible suppression of KRASDepositorInsertshKRAS (KRAS Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1/TOAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPBT-PE2-PuroTK-pegRNA_GG
Plasmid#173222PurposepiggyBac transposon containing both PE2 and a pegRNA cloning site.DepositorInsertsPE2
PuroTK
pegRNA cloning cassette
UsePiggybacTagsExpressionMammalianMutationPromoterCMV, P2A, and hU6Available sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-surfaceHA-IRES-Puro
Plasmid#207321PurposeLentiviral expression of synthetic HA surface receptor in Mammalian cellsDepositorInsertHAtag-Myctag-PDGFRB-TM-IRES-Puro
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alpha_short (EFS)Available sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-NLS-LSSmKate2
Plasmid#34586DepositorInsertNLS-LSS-mKate2
UseRetroviralTagsExpressionMammalianMutationK69Y/P131T/S148G/M167D/T183S/M196V compared to mK…PromoterAvailable sinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
JN302: pMVP/SB/Puro-DEST
Plasmid#121865PurposepMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassetteDepositorTypeEmpty backboneUseSleeping beauty transposon, pmvp gateway destinat…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-HA-UBE3A-IRES-Puro
Plasmid#107349PurposeExpresses HA tagged UBE3A in mammalian cells.DepositorInsertUBE3A (UBE3A Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-mCherry-CAAX PuroR
Plasmid#166228PurposeLentiviral plasmid with Puromycin selection for expression of mCherry with a C-terminal CAAX polybasic sequence from KRras targeting mCherry to the plasma membraneDepositorInsertmCherry-CAAX
UseLentiviralTagsCAAXExpressionMammalianMutationPromoterCMVAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA
Plasmid#196192Purposehuman synapsin promoter driven dTomato expression at human AAVS1 safe harbour locusDepositorInsert2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
UseZnf donorTagsExpressionMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro
Plasmid#28289Purpose3rd generation lentiviral plasmid; This plasmid is the backbone for the DECIPHER libraries (currently DISCONTINUED)DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-MTS-EGFP
Plasmid#190270PurposeLentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cellsDepositorInsertMTS-EGFP
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-MAPRE1
Plasmid#207794PurposeDonor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793DepositorArticleInsertMAPRE1 Homology Arms flanking a moxGFP-Puro Cassette (MAPRE1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO.1 puro shRNA E-cadherin
Plasmid#18801DepositorInsertE-cadherin shRNA (CDH1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceJuly 22, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro /BRAF-V600E
Plasmid#131724PurposeMammalian Expression of Flag tagged BRAFDepositorInsertBRAF (BRAF Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationV600EPromoterCMVAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-PSMD4
Plasmid#202666PurposeTet-ON 3rd generation expression vector with inducible expression of PSMD4 and a puromycin selection cassetteDepositorInsertproteasome 26S subunit ubiquitin receptor, non-ATPase 4 (PSMD4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-JEDI-2P
Plasmid#179458PurposeGenetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)DepositorInsertJEDI-2P
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 hPGK-PuroR-pA donor
Plasmid#22072DepositorInsertPGK-Puro (PGK1 Human)
UseZfn donorTagsExpressionMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBabe(puro) mouse Egr2
Plasmid#108097PurposeRetroviral vector for expression of mouse Egr2DepositorInsertEgr2 (Egr2 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro PPAR gamma 2
Plasmid#8859DepositorInsertPPAR gamma (Pparg Mouse)
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV: Flag-Antares_SV40:PuroR
Plasmid#183049PurposeExpresses FLAG-tagged Antares in mammalian cells.DepositorInsertAntares BRET reporter
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(GATA3(13))-PGKpuro2ABFP-W
Plasmid#208548PurposeLentiviral vector expressing gRNA targeting human GATA3DepositorInsertGATA3(13) (GATA3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(GATA3(12))-PGKpuro2ABFP-W
Plasmid#208547PurposeLentiviral vector expressing gRNA targeting human GATA3DepositorInsertGATA3(12) (GATA3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFH2.155_LV-Puro-T2A-mTagBFP2
Plasmid#205553PurposeExpress puromycin resistance marker and mTagBFP2DepositorInsertPuro-T2A-mTagBFP2
UseLentiviralTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
OCT4-2A-eGFP-PGK-Puro
Plasmid#31938PurposeHuman OCT4 genomic targeting donorDepositorInsertOCT4 (POU5F1 Human)
UseTargeting donorTagseGFPExpressionMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-CMV-VC3AI
Plasmid#78907PurposeIndicator of caspase-3-like protease activityDepositorInsertVC3AI
UseLentiviralTagsHA and MycExpressionMutationS320A, deletion of AA1-200PromoterCMVAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-P112H
Plasmid#133760PurposeLentiviral expression of human TDP-43 P112H disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralTagsExpressionMammalianMutationChanged proline 112 to histidine.PromoterCMVAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro IRES-EGFP
Plasmid#14430DepositorHas ServiceCloning Grade DNAInsertIRES-EGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N-Flag-HA-IRES-PURO
Plasmid#41033DepositorTypeEmpty backboneUseRetroviral; Gateway destination vectorTagsFlag, HA, and IRES PuroExpressionMammalianMutationPromoterLTR-driven expressionAvailable sinceOct. 26, 2012AvailabilityAcademic Institutions and Nonprofits only