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Plasmid#189618PurposeCloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertMitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C4
Plasmid#189621PurposeCloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertReduced Mitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationRepeat region removed, and minor additional mutat…PromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C2
Plasmid#189619PurposeCloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertMitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-tepsin
Plasmid#196647PurposeExpression of GAL4 DNA-binding domain (BD)-tepsin fusion proteinDepositorInserttepsin (TEPSIN Human)
UseYeast two-hybrid vectorTagsGAL4-DNA binding domain fragmentExpressionYeastMutationsilent substitution in codon 199 to eliminate int…PromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
OM14-2EGFP-UG74-ter
Plasmid#184758PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at OM14 C terminus. Uses antibiotic resistance marker natMX6.DepositorInsertOM14 (OM14 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCL.031
Plasmid#184968PurposeTest effect of extending -Eco2 a1/a2 in yeastDepositorInsertEco2: RT and ncRNA(extended), a1/a2 length: 29
UseTagsExpressionYeastMutationhuman codon optimized RT, a1/a2 length extended t…PromoterGal7Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pfa6a mScarlet-FKBP::KANMX
Plasmid#185905PurposePlasmid for cloning mScarlet fused to FKBP into yeast.DepositorInsertmScarlet-FKBP
UseTagsmScarlet fused to FKBPExpressionYeastMutationPromoterSP6Available sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCble
Plasmid#185861PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a phleomycin-resistent selectable markerDepositorInsertPTDH3-Cas9(N); PSNR52-srRNA-TSUP4; ble
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-MTS-GFP11-Ura
Plasmid#177733PurposeEleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged in yeast plasmid with URA markerDepositorInsertMTS-GFP11
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETCON-Aga2p-TAPBPR-myc
Plasmid#153486PurposeYeast expression of Aga2p-TAPBPR-mycDepositorInsertTAPBPR (TAPBPL Human)
UseTagsAga2P and c-myc epitope tagExpressionYeastMutationPromoterGALAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS406 ATG1-ATG7-3'UTR
Plasmid#166847PurposeExpresses Atg1 with ATG7 3'UTR in yeasts.DepositorInsertATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterATG1 PromoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A mmPing20
Plasmid#145788PurposemPing Yeast Transposition AssayDepositorInsertmmPing20
UseTagsExpressionYeastMutationT162C, T258A, T287C, T300A, T304A, T310A, G372APromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A 2xE
Plasmid#145792PurposemPing Yeast Transposition AssayDepositorInsert2xE
UseTagsExpressionYeastMutationInsertion of 2 copies of the CMV EnhancerPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A 4xE
Plasmid#145793PurposemPing Yeast Transposition AssayDepositorInsert4XE
UseTagsExpressionYeastMutationInsertion of 4 copies of the CMV EnhancerPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
Plasmid#140155PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS424-ysg(G:H)
Plasmid#138257PurposeExpression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter systemDepositorInsertGFP targeting sgRNAs G and H
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17(F317D, I325A)
Plasmid#122275PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPhenylalanine 317 to Aspartic acid; Isoleucine 32…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317D, I325A)
Plasmid#122273PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPhenylalanine 317 to Aspartic acid; Isoleucine 32…PromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317D)
Plasmid#121391PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPhenylalanine 317 to Aspartic acidPromoterADH1 promoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(∆335-417)
Plasmid#121525PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationdeleted amino acids 335-417*PromoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only