Addgene

Showing: 5141 - 5160 of 23514 results

Results per page:

Sort results by:

pX459-sg-mGreenLantern/EGFP

Plasmid

#179913

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

Depositor

Johan Sosa, Indeever Madireddy

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern/EGFP sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
His-GFP-Granuphilin

Plasmid

#170867

Purpose

Mammalian expression of His-GFP-Granuphilin.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Synaptotagmin-like 4 (SYTL4 Human)

Use

Tags

(His)6 and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES-hrGFP II-mTET1

Plasmid

#83569

Purpose

Expresses TET1 with a mutated catalytic domain in mammalian cells

Depositor

Jean-Pierre Issa

Article

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Use

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

catalytic domain mutant H1672D, D1674A

Promoter

CMV

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLC-EGFP-RIP3

Plasmid

#75165

Purpose

LentiCRISPR-EGFP with sgRNA targeting human RIP3

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

RIP3 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PKAcs-GGS4X-GFP

Plasmid

#108583

Purpose

Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

Depositor

Robert Hughes

Article

Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

Insert

protein kinase A, catalytic subunit

Use

Tags

GFP

Expression

Mammalian

Mutation

H87Q and W196R

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CB6-GFP-TAOK1

Plasmid

#197108

Purpose

Expression of GFP-tagged TAOK1 / PSK2 in mammalian cells

Depositor

Jonathan Morris

Article

Garg et al J Cell Sci. 2020 Mar 16;133(6):jcs235895

Insert

TAOK1 (TAOK1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Wild Type

Promoter

CMV

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRL.SP146.gp91.eGFP

Plasmid

#30474

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 May 5. ():.

Insert

SP146/gp91/eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-MPro-eGFP-2

Plasmid

#184824

Purpose

Expresses Mpro-eGFP in mammalian cells

Depositor

Wenshe Liu

Article

Cao et al ACS Cent Sci. 2022 Feb 23;8(2):192-204.

Insert

Mpro-eGFP conjugate (M SARS-CoV-2, jellyfish Aequorea Victoria)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1alfa

Available since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-sACE2v2.4-sfGFP

Plasmid

#149666

Purpose

Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2.4

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Linker GSGGSGSGG and superfolder GFP

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-m5HT6-EGFP

Plasmid

#200443

Purpose

Lentiviral expression of mouse m5HT6-EGFP

Depositor

Ken-Ichi Takemaru

Insert

5HT6-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

EF1α

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKar2-msGFP-HDEL

Plasmid

#117668

Purpose

Localisation of Endoplasmic Reticulum

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pKar2-msGFP-HDEL

Use

Tags

Expression

Yeast

Mutation

Contains the Kar2 promoter and signal sequence fr…

Promoter

pKar2

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG304GAL-ccdB-EGFP

Plasmid

#14183

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
YIPlac204TKC-GFP-HDEL

Plasmid

#21771

Depositor

Benjamin Glick

Article

Rossanese et al J Cell Biol. 2001 Apr 2. 153(1):47-62.

Insert

Green Fluorescent Protein (GFP)

Use

Tags

HDEL and preKar2

Expression

Yeast

Mutation

Promoter

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW-NbVHH05-GFP

Plasmid

#171570

Purpose

Expresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cells

Depositor

Hidde Ploegh, Norbert Perrimon

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-GFP (UBE2J1 Human)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

fly actin5C promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCBB-pT7-eGFP

Plasmid

#32552

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Mutation

Promoter

pT7

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pBFv-UAS3-GFP1-10

Plasmid

#138398

Purpose

Expresses split GFP fragment (GFP1-10)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

GFP1-10

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAd NeuroD-I-nGFP

Plasmid

#19414

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Aug 27. ():.

Insert

neurogenic differentiation 1 (Neurod1 Mouse)

Use

Adenoviral; Entry vector

Tags

GFP and IRES

Expression

Mutation

Promoter

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
rTetR-GFP-VP16

Plasmid

#183917

Purpose

VP16 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

rTetR-GFP-VP16 (UL48 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG413GAL-ccdB-EGFP

Plasmid

#14189

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE GFP-CACNA1E KI

Plasmid

#131481

Purpose

Endogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacna1e, Cacna1e Rat, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge