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Plasmid#204339PurposeGPCR/G protein BRET biosensorDepositorInsertGbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-B-Seipin-miniIAA7-mEGFP
Plasmid#129719PurposeAAVS1 safe harbor targeting vector expressing miniIAA7-mEGFP tagged SeipinDepositorInsertSeipin (BSCL2 Human)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsminiIAA7-mEGFPExpressionMammalianMutationsilent mutationsPromoterEF1aAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-dCasRx-RBM25-IRES-BleoR
Plasmid#221002PurposeStable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.DepositorInsertdCasRx-RBM25-IRES-BleoR
UseCRISPRTagsNLS and NLS-HAExpressionMammalianMutationPromoterCAGAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-hygro-EGFP-DFCP1
Plasmid#174651PurposeVector for overexpression of EGFP-tagged DFCP1 with IRES for hygromycin selection.DepositorInsertEGFP-DFCP1 (ZFYVE1 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterCMVAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-IgA1-IRES-GFP
Plasmid#187007PurposeExpression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1DepositorInsertHuman anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOTTC385 - pAAV CMV-IE IRES EGFP
Plasmid#102936PurposeAn AAV vector that expresses IRES EGFP under the CMV-IE promoterDepositorInsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterCMV-IEAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-CDK19-WT-IRES-ZsGreen
Plasmid#68858PurposeExpresses Human CDK19 Wild-TypeDepositorInsertCyclin-Dependent Kinase 19 (CDK19 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1αAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWPI-IRES-Bla-Ak-ACE2-TMPRSS2
Plasmid#154983PurposeLentiviral vector expressing Human ACE2 and TMPRSS2DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR
Plasmid#171594PurposeStrong human ACE2 abundance from Bxb1 landing padDepositorInsertsUseSynthetic Biology; Promoterless bxb1 dna recombin…TagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-mKate2-3xNLS-IRES-Pac
Plasmid#183618PurposeMammalian expression vector for mKate2-3xNLS from the CAG promoter. Confers puromycin resistance.DepositorInsertmKate2-3xNLS
UseTags3xNLSExpressionMammalianMutationPromoterCAGAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-hSNCA
Plasmid#185715PurposeAAV expression of GFP and human α-Synuclein from hSyn promoterDepositorInsertsynuclein, alpha (SNCA Human)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
MMLV-CAG-TVA-IRES-Puro
Plasmid#172366PurposeGeneration of TVA-expressing stable cell lines, resistant to the antibiotic PuromycinDepositorInsertTVA + Puro
UseRetroviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS306-URA3-PHIS3-AqLumSynth –Sapphire
Plasmid#116931PurposeYeast integrative vector for the exression of the 20nm GEM under the HIS3 promoterDepositorInsertlumazine synthase
UseTagsSapphireExpressionYeastMutationPromoterHIS3Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human HoxB4-IRES-YFP
Plasmid#91889PurposeRetroviral expression of human HoxB4DepositorInsertHoxB4 (HOXB4 Human)
UseRetroviralTagsIRES-EYFPExpressionMammalianMutationPromoterMMLV gagAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-IgM-IRES-GFP
Plasmid#187011PurposeExpression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgMDepositorInsertHuman anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRE-KRAB-dCas9-IRES-GFP
Plasmid#85556Purpose2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFPDepositorInsertKRAB-dCas9-IRES-GFP
UseCRISPR and LentiviralTags2x NLS, HA Tag, and KRAB domainExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-IgG1-IRES-GFP
Plasmid#187008PurposeExpression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1DepositorInsertHuman anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRRL-TetO-Pax7-IRES-EGFP
Plasmid#109372PurposeExpresses Dox-inducible Pax7 together with a GFP reporterDepositorInsertPax7
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-CDH5-mRuby3-IRES-puro
Plasmid#85144Purposelentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3DepositorInsertCDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)
UseLentiviralTagsmRuby3ExpressionMutationPromoterCAGAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shSCR-Puro
Plasmid#135689PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Puromycin selectionDepositorInsertshScrambled
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Oct4-IRES-eGFP-PGK-Neo
Plasmid#48681PurposeFor knock-in donor vector by CRISPR/CAS systemDepositorInsertOct4 (Pou5f1 Mouse)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-CDK8-WT-IRES-mCherry
Plasmid#68856PurposeExpresses Human CDK8 Wild-TypeDepositorInsertcyclin-dependent kinase 8 (CDK8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1αAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 blast OFF 3xflag IREB2
Plasmid#169889PurposeExpress 3x flag IREB2, suppressible by doxycycline additionDepositorInsertIREB2 (IREB2 Human)
UseLentiviral; Doxycycline mediated suppressionTags3x FlagExpressionMammalianMutationSilent mutations in sgIREB2_1 and sgIREB2_2 targe…PromoterTREAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human Igf2bp2-IRES-GFP
Plasmid#91890PurposeRetroviral expression of human Igfbp2DepositorInsertIgf2bp2 (IGF2BP2 Human)
UseRetroviralTagsIRES-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-human-Caspase-1
Plasmid#183359PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1 (CASP1 Human)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFP5380 LAMP1-SBP- mCherry-FIREtag
Plasmid#216725PurposeExpresses LAMP1-mCherry-FIREtag in mamallian cellsDepositorInsertLAMP-1 (LAMP1 Human)
UseTagsFIREtag, Streptavidin Binding Protein (SBP), and …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB-IRES-copGFP-T2A-Puro
Plasmid#72299PurposeExpress gene of interest under CB promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterchicken beta-actin + CMV enhancerAvailable sinceJan. 25, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG1345 mCherry-FIREtag-DEVDG-MoA
Plasmid#205176PurposeExpresses mCherry-FIREtag-DEVDG-MoA in mammalian cellsDepositorInsertmCherry-cagedFIREtag
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine
Plasmid#50470PurposeGq-coupled hM3D-IRES-mCitrine under the control of GFAP promoterDepositorInserthM3D(Gq)-IRES-mCitrine (CHRM3 Human)
UseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-N2c_envA-IRES-Neo
Plasmid#172368PurposeGeneration of envA-expressing stable cell lines, resistant to the antibiotic Neomycin (Gentamicin/G418)DepositorInsertenvA + Neo
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-MPAct-mCit-IRES-Neo
Plasmid#155230Purposelentiviral MPAct constructDepositorInsertLentiviral expression of MPAct-mCitrine sensor
UseLentiviralTagsmCitrineExpressionMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mNG-IRES-mScarlet-dPCP
Plasmid#231090PurposeBicistronic construct encoding mNG in combination with mScarlet-dPCP via an EMCV-IRES sequence.DepositorInsertmNG-IRES-mScarlet-dPCP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD
Plasmid#169818PurposeExpresses C-terminal flag-tagged CAD in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationTCCC -> AGTC silent mutations at nt527-530 eli…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine
Plasmid#50463PurposeGq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoterDepositorInserthM3D(Gq)-IRES-mCitrine (CHRM3 Human)
UseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCV-AML1/ETO-IRES-GFP
Plasmid#60832PurposeAML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFPDepositorUseRetroviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IRES-blast GFP-SPNS1
Plasmid#198571PurposeSPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vectorDepositorInsertSPNS1 with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
UseRetroviralTags3xFlag-GFPExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-CMV-DsRed-IRES-d4EGFP
Plasmid#41943PurposeRetroviral expression of DsRed in mammalian cellsDepositorInsertDsRed
UseRetroviralTagsExpressionMammalianMutationPromoterTREAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-Caspase-1 (C285A)
Plasmid#131353PurposeExpresses Caspase-1 C285ADepositorInsertCaspase-1 (C285A) (Casp1 Mouse)
UseRetroviralTagsExpressionMammalianMutationCaspase-1 (C285A)PromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-mouse-Caspase-11
Plasmid#183358PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-11 (Casp4 Mouse)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
hMYOD1-3xHA-IRES-hrGFP (#343)
Plasmid#78327PurposeOverexpression of human MYOD1 ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporterDepositorInsertMYOD1 (MYOD1 Human)
UseTags3x HA tag and IRES-hrGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-MinE-EGFP_IRES-Blast
Plasmid#213113PurposeExpression of MinE-EGFP in mammalian cellsDepositorInsertMinE-EGFP (minE Escherichia coli)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB CAG Otx2-IRES-EGFP
Plasmid#153947PurposepiggyBac transposon vector with CAG promoter expressing Otx2 and EGFPDepositorInsertOtx2 (Otx2 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV Fcgamma RIIa IRES neo
Plasmid#14948DepositorInsertFcgamma RIIa (FCGR2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
MSCV-CMV-DsRed-IRES-d24EGFP
Plasmid#41944PurposeRetroviral expression of DsRed in mammalian cellsDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-mRuby3-MLC-IRES-Neo
Plasmid#222635PurposeReporter for activated NM2DepositorInsertmRuby3-MLC (MYL9 Human)
UseLentiviralTagsmRuby3ExpressionMutationPromoterEF1aAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-NE2m-IRES-mCherry-CAAX
Plasmid#208690PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cellsDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2m
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-Seipin-miniIAA7-mScarlet-I
Plasmid#129721PurposeAAVS1 safe harbor targeting vector expressing miniIAA7-mScarlet-I tagged SeipinDepositorInsertSeipin (BSCL2 Human)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsminiIAA7-mScarlet-IExpressionMammalianMutationsilent mutationsPromoterEF1aAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac-FLAG-mProser1-IRES-Blast
Plasmid#226172PurposeExpresses full length mouse PROSER1 with 2X N-terminal FLAG tagsDepositorInsertProline Serine Rich protein 1 (Proser1 Mouse)
UseTags2X FLAGExpressionMammalianMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR
Plasmid#190077Purposeexpress human ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance geneDepositorUseTagsExpressionMammalianMutationPromoternoneAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Ace2.IRES-puro.WPRE (MT126)
Plasmid#145839PurposeLentiviral vector genome to ectopically express human Ace2DepositorInserthuman Ace2 (ACE2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only