pLVX-IRES-hygro-EGFP-DFCP1
(Plasmid
#174651)
-
PurposeVector for overexpression of EGFP-tagged DFCP1 with IRES for hygromycin selection.
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174651 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepLVX
-
Vector typeLentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameEGFP-DFCP1
-
Alt nameZFYVE1, PPP1R172, SR3, TAFF1, ZNFN2A1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)3087
-
Entrez GeneZFYVE1 (a.k.a. DFCP1, PPP1R172, SR3, TAFF1, ZNFN2A1)
- Promoter CMV
-
Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Internal plasmid ID: AJ212 bioRxiv 2021.04.21
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLVX-IRES-hygro-EGFP-DFCP1 was a gift from Lukas Kapitein (Addgene plasmid # 174651 ; http://n2t.net/addgene:174651 ; RRID:Addgene_174651) -
For your References section:
Direct observation of aggregate-triggered selective autophagy in human cells. Janssen AFJ, Korsten G, Nijenhuis W, Katrukha EA, Kapitein LC. J Cell Sci. 2021 Oct 1;134(19). pii: 271972. doi: 10.1242/jcs.258824. Epub 2021 Oct 6. 10.1242/jcs.258824 PubMed 34447998
