Addgene

Showing: 4781 - 4800 of 11721 results

Results per page:

Sort results by:

pRS314-prp16-1

Plasmid

#89972

Purpose

prp16-1 allele in TRP plasmid

Depositor

Christine Guthrie

Article

Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

Insert

PRP16 (PRP16 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

prp16-1 allele (Y386D)

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS314-prp16-101

Plasmid

#89973

Purpose

prp16-101 allele in TRP plasmid

Depositor

Christine Guthrie

Article

Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

Insert

PRP16 (PRP16 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

prp16-101 allele (G373S)

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
CaMCLC-His

Plasmid

#111292

Purpose

bacterial expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

(S)-malyl-CoA/b-methylmalyl-CoA/(S)-citramalyl- CoA lyase

Use

Tags

His tag, GGGSGGGSLEHHHHHH

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
MsMUT_sgRNA1

Plasmid

#111296

Purpose

CRISPR KO murine MUT

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA1 against murine MUT

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
MsMUT_sgRNA3

Plasmid

#111297

Purpose

CRISPR KO murine MUT

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA3 against murine MUT

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
NaVSp1 FL DeltaCTD pIRES

Plasmid

#128077

Purpose

Mammalian cell expression vector for NaVSp1 bacterial sodium channel. Residues 1-224.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVSp1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
NaVAe1 FL DeltaCTD pIRES

Plasmid

#128078

Purpose

Mammalian cell expression vector for NaVAe1 bacterial sodium channel. Residues 1-241.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVAe1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
NaVAe1 DeltaCC pIRES

Plasmid

#128086

Purpose

Mammalian cell expression vector for NaVAe1 1-264 chimera bacterial sodium channel.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVAe1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL3 - Brr2 del113 N1104L

Plasmid

#90253

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Use

Tags

TAP

Expression

Yeast

Mutation

deleted first 113 amino acids and amino acid N110…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL7 - Brr2 del247 N1104L

Plasmid

#90257

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Use

Tags

TAP

Expression

Yeast

Mutation

deleted first 247 amino acids and N1104L

Promoter

Available since

Availability

Academic Institutions and Nonprofits only
pSL11 - Brr2 del422 N1104L

Plasmid

#90261

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Use

Tags

TAP

Expression

Yeast

Mutation

deleted first 422 amino acids and amino acid N110…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL12 - Brr2 del422 Q904E

Plasmid

#90262

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Use

Tags

TAP

Expression

Yeast

Mutation

deleted first 422 amino acids and amino acid Q904E

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL13 - Brr2 del422 R1107L

Plasmid

#90263

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Use

Tags

TAP

Expression

Yeast

Mutation

deleted first 422 amino acids and amino acid R110…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
WT Ecm2

Plasmid

#169922

Purpose

Ecm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

Ecm2

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
enz.043-SETD7-Y305F

Plasmid

#154045

Purpose

Expresses SETD7 (Y305F) in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

Histone-lysine N-methyltransferase SETD7 (SETD7 Human)

Use

Tags

His, MBP

Expression

Bacterial

Mutation

changed tyrosine 305 to phenylalanine

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pSOUP

Plasmid

#165419

Purpose

The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

Depositor

John Innes Centre - Crop Transformation and Genome Editing Group, Wendy Harwood

Article

Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

Insert

Rep A

Use

Helper plasmid required for replication for all p…

Tags

Expression

Mutation

Promoter

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Mut2-RS

Plasmid

#182882

Purpose

A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

Depositor

Miriam Amiram

Article

Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384

Insert

Mut2-RS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FlagMBNL3

Plasmid

#96901

Purpose

expression of Flag-tagged human MBNL3

Depositor

Thomas Cooper

Article

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

human MBNL3 cDNA (MBNL3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSETB/mStayGold

Plasmid

#212017

Purpose

mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

mStayGold

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX303-ZIM3-KRAB-dCas9

Plasmid

#154472

Purpose

Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

Depositor

Mikko Taipale

Article

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Synthetic)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge