-
Plasmid#21711DepositorInsertROSA26 G19 (Gt(ROSA)26Sor Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGlul
Plasmid#119832PurposeProduction of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGlul
UseTagsGSTExpressionBacterialMutationT92APromoterT7Available sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
M49 GST-TEV-Chibby-C
Plasmid#17197DepositorInsertChibby (CBY1 Human)
UseTagsGSTExpressionBacterialMutationamino acids 64-126PromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
CD23b in pIRES2eGFP
Plasmid#19136DepositorInsertCD23 beta (FCER2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBT296_pBS-U-attP-FRT5-pSV40-Neo-pA-FRT5
Plasmid#52537PurposeExpression of FRT5-flanked Neo; homologous recombination targeting vector cloningDepositorInsertsPhiC31o attP (full length)
Neo
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
EGFP-bSV1_1-1009
Plasmid#129471PurposeMammalian expression of the N-terminus + central domain of bovine SV1DepositorInsertSupervillin (SVIL Bovine)
UseTagsExpressionMammalianMutationcontains amino acids 1-1009PromoterCMVAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT298_pBS-U-attPx3-FRT5-pSV40-Neo-pA-FRT5
Plasmid#52538PurposeExpression of FRT5-flanked Neo; homologous recombination targeting vector cloningDepositorInsertsPhiC31o attPx3 (70 bp each)
Neo
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOCC81
Plasmid#118900Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C , and N-terminal GSTDepositorInsertNcoI-HIS6-3C-GST-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease; GST tagExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 D444-500
Plasmid#19839DepositorInsertMKL1 D444-500 (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationdeleted amino acids 444-500PromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pOCC6
Plasmid#118864Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal EGFPDepositorInsertNcoI-HIS6-3C-EGFP-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease, EGFPExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIS1 DICER1 short UTR
Plasmid#21648DepositorInsertDicer 1 (DICER1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
TR-5'UTR-HP-GFP
Plasmid#80594PurposeExpresses GFP from the CBA promoter. Includes a Csy4 target hairpin in the 5'UTR for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertGFP
UseAAVTagsExpressionMammalianMutationInserted 30 nt hairpin which is targeted by Csy4 …PromoterCBAAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA6/ERGIC2-WT
Plasmid#51479PurposeExpresses ERGIC2-WT in mammalian cellsDepositorInsertERGIC2-WT (ERGIC2 Human)
UseTagsnoneExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 D500-630
Plasmid#19841DepositorInsertMKL1 D500-630 (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationdeleted amino acids 500-630PromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ERKsubDD-Venus-NES
Plasmid#84630PurposeEncodes C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorInsertERKsubDD-Venus-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW2
Plasmid#166708Purposeexpresses NEDD4L with inactivating mutation in second WW domain in mammalian cellsDepositorInsertNEDD4L W453A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 453 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW3
Plasmid#166709Purposeexpresses NEDD4L with inactivating mutation in third WW domain in mammalian cellsDepositorInsertNEDD4L W525A
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 525 to alaninePromoterCMV promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
gamma2 CR shRNA (shRNA2)
Plasmid#120794PurposeshRNA targeting the coding region of the gamma2 mRNADepositorInsertGABRG2 shRNA (Gabrg2 Rat)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMU103
Plasmid#73934PurposeThis is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.DepositorInsertbar and GUS cassettes
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only