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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 21899 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Tetracycline, 100 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B/P3
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Growth instructionsneeds P3-containing host strain such as DH10B/P3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCHOP10
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SpeciesM. musculus (mouse)
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Entrez GeneDdit3 (a.k.a. AltDDIT3, CHOP-10, CHOP10, chop, gadd153)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note that the phagemid had a scrambled insert and the portion 3' of the CHOP-10 coding region (in red-see author's map) is not from the CHOP gene. If you wish to obtain a CHOP probe for hybridization experiments we suggest excising the HD3-Nhe1 fragment that contains CHOP coding sequence.
Note that this plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCHOP10.pCDNA1 was a gift from David Ron (Addgene plasmid # 21899 ; http://n2t.net/addgene:21899 ; RRID:Addgene_21899) -
For your References section:
CHOP, a novel developmentally regulated nuclear protein that dimerizes with transcription factors C/EBP and LAP and functions as a dominant-negative inhibitor of gene transcription. Ron D, Habener JF. Genes Dev. 1992 Mar;6(3):439-53. doi: 10.1101/gad.6.3.439. 10.1101/gad.6.3.439 PubMed 1547942
Map uploaded by the depositor.