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NW9

Plasmid

#133442

Purpose

Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pYZ301

Plasmid

#102690

Purpose

S. pombe gRNA entry vector with Cas9 - Sp lys9 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ294

Plasmid

#102688

Purpose

S. pombe gRNA entry vector with Cas9 - KanMX marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ300

Plasmid

#102689

Purpose

S. pombe gRNA entry vector with Cas9 - Sp his3 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
NW30

Plasmid

#133444

Purpose

Covalently circularized nanodisc NW30 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW30

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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NW50

Plasmid

#133445

Purpose

Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW50

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ293

Plasmid

#102687

Purpose

S. pombe gRNA entry vector with Cas9 - Sp leu1 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR SD Age HsSAS-6

Plasmid

#46381

Purpose

For expression of C-terminal fusions.

Depositor

Pierre Gonczy

Article

Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

Insert

SAS-6 (SASS6 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B2 WT

Plasmid

#123075

Purpose

Gateway entry clone encoding human MAP1LC3B2 wild-type

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRST11B-AS3_4

Plasmid

#102789

Purpose

Expresses tryptophanyl tRNA synthetase (ScWRS) and supressor tryptophanyl tRNA

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Inserts

tryptophanyl tRNA synthetase (WRS1 Budding Yeast)
suppressor tRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 3, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pC-PTP-NEO

Plasmid

#51710

Purpose

contains a C-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

Depositor

Arthur Gunzl

Article

Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

Type

Empty backbone

Use

T. brucei genome integration vectors

Tags

PTP (ProtC-TEV-ProtA)

Expression

Mutation

Promoter

Available since

March 12, 2014

Availability

Academic Institutions and Nonprofits only

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pN-PURO-PTP

Plasmid

#51711

Purpose

contains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

Depositor

Arthur Gunzl

Article

Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

Type

Empty backbone

Use

T. brucei genome integration vectors

Tags

PTP (ProtC-TEV-ProtA)

Expression

Mutation

Promoter

Available since

March 19, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR Age HsSAS-6 ST

Plasmid

#46382

Purpose

For expression of N-terminal fusions.

Depositor

Pierre Gonczy

Article

Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

Insert

SAS-6 (SASS6 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pIM43

Plasmid

#193537

Purpose

pENTRY clone for NLR Integrated Domain ID#78

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#78

Use

Pentry clone

Tags

Expression

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pIM63

Plasmid

#193545

Purpose

pENTRY clone for NLR Integrated Domain ID#19

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#19

Use

Pentry clone

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pIM68

Plasmid

#193550

Purpose

pENTRY clone for NLR Integrated Domain ID#50

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#50

Use

Pentry clone

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pIM42

Plasmid

#193536

Purpose

pENTRY clone for NLR Integrated Domain ID#58

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#58

Use

Pentry clone

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pIM65

Plasmid

#193547

Purpose

pENTRY clone for NLR Integrated Domain ID#14

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#14

Use

Pentry clone

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pIM46

Plasmid

#193540

Purpose

pENTRY clone for NLR Integrated Domain ID#89

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#89

Use

Pentry clone

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pIM37

Plasmid

#193531

Purpose

pENTRY clone for NLR Integrated Domain ID#63

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#63

Use

Pentry clone

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge