-
Plasmid#123201PurposeWe have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.DepositorInsertSiriusGFP
UseMouse TargetingTagsN/AExpressionBacterial and MammalianMutationeGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…PromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E_BAG3wt-eGFP
Plasmid#98180PurposeCodes for full length, GFP tagged, human wildtype Bag3DepositorInsertBAG3wt-eGFP (BAG3 Human)
UseGateway vectorTagsGFPExpressionMutationPromoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-pAce-Kv2.1PR
Plasmid#195523PurposeGreen fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targetedDepositorInsertpAce-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 78K, 81D, 92N, 178F; SY linkerPromoterSynapsinAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2 sgNRF2-3
Plasmid#186838Purposeknock out NRF2 in mammalian cellsDepositorInsertNrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
PINK1 gRNA (BRDN0001147554)
Plasmid#78035Purpose3rd generation lentiviral gRNA plasmid targeting human PINK1DepositorInsertPINK1 (PINK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
PINK1 gRNA (BRDN0001145357)
Plasmid#78036Purpose3rd generation lentiviral gRNA plasmid targeting human PINK1DepositorInsertPINK1 (PINK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS306-URA3-PHIS3-AqLumSynth –Sapphire
Plasmid#116931PurposeYeast integrative vector for the exression of the 20nm GEM under the HIS3 promoterDepositorInsertlumazine synthase
UseTagsSapphireExpressionYeastMutationPromoterHIS3Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV ORANGE Dlg4-HaloTag KI
Plasmid#139656PurposeEndogenous tagging of PSD95: C-terminal (amino acid position: R721)DepositorInsertgRNA and HaloTag donor
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-dLight1.3b
Plasmid#135762PurposeFor expression of the genetically encoded dopamine sensor dLight1.3bDepositorHas ServiceAAV9InsertdLight1.3b
UseAAVTagsFlag tagExpressionMammalianMutationPromoterSynapsinAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCTIP2-N106
Plasmid#66808Purpose2nd generation lentiviral transfer plasmid. Expresses mouse CTIP2 with blasticidin resistanceDepositorInsertCTIP2 isoform b (Bcl11b Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterHuman EF1aAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-NS1NF
Plasmid#175279PurposeAAV vector mediating inducible expression of the NS1 gene of YFV-17DDepositorInsertNS1 (POLY Yellow fever virus strain 17D)
UseAAVTagsExpressionMutationPromotertight TREAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO-RB1-shRNA63
Plasmid#25641Purpose3rd generation lentiviral vectorDepositorInsertRB1-shRNA 63 (RB1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Atg14L
Plasmid#21635DepositorInsertAtg14L (ATG14 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hnRNPU-V5
Plasmid#35974DepositorInsertheterogeneous nuclear ribonucleoprotein U (HNRNPU Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-M13-TevC-IRES-SP6-Cre
Plasmid#171620PurposeM13-TevC with CreDepositorInsertM13-TevC-IRES-SP6-Cre
UseAAV and Synthetic BiologyTags6x HisExpressionMammalianMutationPromoterhSynAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-pAceR-Kv2.1PR
Plasmid#195534PurposeCre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targetedDepositorInsertpAceR-Kv2.1 proximal restriction sequence
UseAAV and Cre/LoxTagsExpressionMammalianMutationVARNAM 78E, 81D, 92N; SI linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenLox_U6 p53
Plasmid#59360PurposeLentiviral expression vector: p53 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.DepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterCAG and mouse U6Available sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
lentiCRISPR v2 sgNRF2-4
Plasmid#186839Purposeknock out NRF2 in mammalian cellsDepositorInsertNrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA
Plasmid#37090PurposeExpresses humanized ChR2(H134R)-mCherry in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitationDepositorInserthChr2(H134R)-mCherry
UseAAV and Cre/Lox; Cre-offTagsmCherryExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin- soCoChR-GFP
Plasmid#107708PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoterDepositorHas ServiceAAV9InsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianMutationPromoterhSynapsinAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DYRK2-2A-mCherry
Plasmid#118285PurposeExpresses mouse DYRK2 tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.DepositorInsertdual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (Dyrk2 Mouse)
UseAAVTags2A peptide and mCherryExpressionMammalianMutationPromoterCMV with beta global intronAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
LLP339 pGK-dCas9-SunTag
Plasmid#100943PurposedCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1aDepositorInsertdCas9, SunTag array (10xGCN4)
UseCRISPRTags3xHAExpressionMammalianMutationD10A, H840A for dCas9PromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-cofilin-S3A
Plasmid#78296PurposeMammalian expression of mouse cofilin S3A (nonphosphorylatable form) fused to EGFPDepositorInsertcofilin 1 (Cfl1 Mouse)
UseTagsEGFPExpressionMammalianMutationS3APromoterCAGAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
PINK1 gRNA (BRDN0001487057)
Plasmid#78037Purpose3rd generation lentiviral gRNA plasmid targeting human PINK1DepositorInsertPINK1 (PINK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS306-URA3-PHIS3-PfV-GS-Sapphire
Plasmid#116932PurposeYeast integrative vector for the exression of the 40nm GEM under the HIS3 promoterDepositorInsertPfv
UseTagsSapphireExpressionYeastMutationPromoterHIS3Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJ179
Plasmid#85996PurposeOne-guide Perturb-seq vector backbone; modified mouse U6 promoter; constant region 2DepositorInsertsEGFP-NT2_cr2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermodified mouse U6Available sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-SPOTlight-U2GCR
Plasmid#164819PurposeReporter to measure ISR state-dependent protein synthesisDepositorInsertSPOTlight-U2GCR
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TLR targeting vector
Plasmid#64215PurposeTargeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) constructDepositorInserttraffic light reporter (TLR) (AAVS1 Human)
UseHuman targetingTagsExpressionMutationsee publication for detailsPromoterCAGAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMJ117
Plasmid#85997PurposeOne-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3DepositorInsertsEGFP-NT2_cr3
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermodified human U6Available sinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001487130)
Plasmid#80262Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
NLS-Laconic/pCMV-myc-nuc
Plasmid#46307Purposea nuclear-targeted, genetically-encoded sensor for lactateDepositorInsertNLS-Laconic
UseLactate biosensorTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHD-SPARC2-S-LexA::p65
Plasmid#133564PurposeSwap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locusDepositorInsertLexA::p65
UseCRISPRTagsExpressionInsectMutationPromoter20X UASAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
Lentiviral-His-tagged-PSPH
Plasmid#134786PurposeStable overexpression lentiviral vector of His tagged PSPHDepositorInsertPhosphoserine phosphatase (PSPH Human)
UseLentiviralTagsHisExpressionMutationPromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTet-GLI2shR
Plasmid#136691PurposeInducible Gli2 shRNA lentiviral plasmid (target sequence: human GLI2 5′CCGGCCTGGCATGACTACCACTATGCTCGAGCATAGTGGTAGTCATGCCAGGTTTTTG 3′)DepositorInsertshRNA_humanGli2 (GLI2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-4mtD3cpv
Plasmid#181874PurposeExpresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoterDepositorInsert4mtD3cpv
UseAAV and AdenoviralTagsExpressionMammalianMutationthe 4mtD3cpv CDS differs from the recorded GeneBa…PromoterCaMKIIaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe EGFR(L858R/T790M)
Plasmid#32073DepositorInsertEGFR (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationL858R/T790MPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
Mito-mGreenLantern
Plasmid#164461PurposeLocalization of mGreenLantern fluorescent protein to mitochondriaDepositorInsertmGreenLantern
UseTagsHuman COX8a first 29 amino acidsExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-G88P3-HA-hM3Dq
Plasmid#213972PurposeAAV vector with G88P3 promoter that can drive robust gene expression in MSNsDepositorInsertG88P3
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-Meis1
Plasmid#139824Purposedoxycycline-inducible expression of mouse Meis in mammalian cellsDepositorInsertMeis1 (Meis1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE-mCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Synapsin-pHoenix-WPRE
Plasmid#70111Purposelentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesiclesDepositorInsertpHoenix (Syp Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat)
UseLentiviralTagsArch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…ExpressionMutationPromoterSynapsinAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBMN_HA-TBK1 (kinase dead - D135N)
Plasmid#199206PurposeUsed for the expression of TBK1 carrying a kinase dead mutation D135N (SMC Internal No. 1997)DepositorInsertTBK1 (TBK1 Human)
UseRetroviralTagsExpressionMammalianMutationD135NPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
TPC1-mCherry
Plasmid#135182PurposeExpresses TPC1 with C-terminus mCherry tag in mammalian cellsDepositorInsertTPC1 (Tpcn1 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEB31 (hANGPTL8-V5-His lenti)
Plasmid#109116PurposeV5 and His-tagged human ANGPTL8 in a lentiviral vectorDepositorInsertANGPTL8 (ANGPTL8 Human)
UseLentiviralTags6x His and V5ExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-pAce-Kv2.1PR
Plasmid#195529PurposeGreen fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targetedDepositorInsertpAce-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 78K, 81D, 92N, 178F; SY linkerPromoterCaMKIIAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BLRR
Plasmid#158958PurposeBioluminescent repair reporter (BLRR) for DNA double strand break (DSB) repairs: homology-directed repair (HDR) and non-homologous end joining (NHEJ).DepositorInsertBLRR
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSI-Target-AID-NG
Plasmid#119861PurposeExpresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.DepositorInsertnSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Target-ACEmax
Plasmid#139105PurposeMammalian Target-ACEmax expression vector (pSI-625)DepositorInsertBi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Rabbit GFP Cav 2.2 pMT2
Plasmid#58737Purposeexpression of GFP tagged rabbit Cav2.2 in mammalian cellsDepositorInsertCav2.2 (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationPromoterSV40Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only