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Plasmid#193219PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Mroh2b geneDepositorInsertsgMroh2b#1 (Heatr7b2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-G60A)
Plasmid#176144PurposeEGFP fused to the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Gly60Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassetteDepositorInsertPolB-T2A-SIRT6 (POLB Human)
UseLentiviralTagsEGFPExpressionMammalianMutationMutation in Gly60 to Ala, and a mutation in the P…PromoterEF1AAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-R65A)
Plasmid#176143PurposeEGFP fused the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Arg65Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassetteDepositorInsertPolB-T2A-SIRT6 (POLB Human)
UseLentiviralTagsEGFPExpressionMammalianMutationMutation in Arg65 to Ala, and a mutation in the P…PromoterEF1AAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
PCIN4-PURO-K8
Plasmid#44626DepositorInsertK8 (KRT8 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-HAPLN1
Plasmid#200777PurposeExpresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoterDepositorInsertHyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
UseAAVTagscfGFPExpressionMutationPromoterSynapsinAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-∆Hapln1
Plasmid#200778PurposeExpresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoterDepositorInsertHyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
UseAAVTagscfGFPExpressionMutationdeleted lectican binding domain (aminoacids 40-15…PromoterSynapsinAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgCrebbp#2/Cre
Plasmid#193210PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp geneDepositorInsertsgCrebbp#2 (Crebbp Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 KSR2
Plasmid#25968DepositorInsertKinase suppressor of Ras 2 (Ksr2 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS/XBB.1.5-RBD
Plasmid#213038PurposeMammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variantDepositorInsertpCAGGS/XBB.1.5-RBD (S )
UseTags6 His TagExpressionMammalianMutationG339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Omicron.BA.5-RBD
Plasmid#213478PurposeMammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variantDepositorInsertpCAGGS/Omicron.BA.5 RBD (S )
UseTags6 His TagExpressionMammalianMutationG339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS/EG5-RBD
Plasmid#213410PurposeMammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.EG5 variantDepositorInsertpCAGGS/EG5-RBD (S )
UseTags6 His TagExpressionMammalianMutationG339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-P1-like
Plasmid#173789PurposeMammalian expression of SARS-CoV-2 Spike protein P-1-like variant (K417N)DepositorInsertSpike S-GSAS- P-1-like variant (K417N) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV KSR2 IRES GFP
Plasmid#25969DepositorInsertKinase suppressor of Ras 2 (Ksr2 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-B.1.351.v6
Plasmid#173791PurposeMammalian expression of SARS-CoV-2 Spike protein South African variant version 6DepositorInsertSpike (S-GSAS-B.1.351 variant version 6) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-deltaH69/V70
Plasmid#171744PurposeMammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-deltaH69/V70 variantDepositorInsertSpike (S-GSAS-D614G-deltaH69/V70 variant) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-B.1.351.v5
Plasmid#173790PurposeMammalian expression of SARS-CoV-2 Spike protein South African variant version 5DepositorInsertSpike (S-GSAS-B.1.351 variant version 5) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-B.1.351.v3
Plasmid#173787PurposeMammalian expression of SARS-CoV-2 Spike protein South African variant version 3DepositorInsertSpike (S-GSAS-B.1.351 variant version 3) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-D614G-N501Y
Plasmid#171748PurposeMammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-N501Y variantDepositorInsertSpike (S-GSAS-D614G-N501Y variant) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-D614G-E484K
Plasmid#171751PurposeMammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-E484K variantDepositorInsertSpike (S-GSAS-D614G-E484K variant) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJuly 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-D614G-K417N
Plasmid#171752PurposeMammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-K417N variantDepositorInsertSpike (S-GSAS-D614G-K417N variant) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJuly 16, 2021AvailabilityAcademic Institutions and Nonprofits only