AAV-HAPLN1
(Plasmid #200777)

• Purpose: Expresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoter
• Depositing Lab: Alexander Dityatev
• Publication: Ramsaran et al Science. 2023 May 5;380(6644):543-551. doi: 10.1126/science.ade6530. Epub 2023 May 4.

Backbone

• Vector backbone: pAAV-Syn_MCS_WPRE_Sv40pA
• Backbone manufacturer: VectorBuilder
• Backbone size w/o insert (bp): 4949
• Total vector size (bp): 6647
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Hyaluronan And Proteoglycan Link Protein 1
• Alt name: HAPLN1
• Species: M. musculus (mouse)
• Insert Size (bp): 1068
• Entrez Gene: Hapln1 (a.k.a. CLP, Crtl1, Crtl1l, LP, LP-1)
• Promoter: Synapsin
• Tag / Fusion Protein:
  • cfGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: GCCGCCTCAGCACTGAAG
• 3′ sequencing primer: ACTTGTGGCCGTTTACGTC

Resource Information

• Supplemental Documents:
  • AAV-HAPLN1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry