-
Plasmid#78297PurposeMammalian expression of mouse cofilin S3D fused to EGFPDepositorInsertcofilin 1 (Cfl1 Mouse)
UseTagsEGFPExpressionMammalianMutationS3DPromoterCAGAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Bsn KI
Plasmid#139664PurposeEndogenous tagging of Bassoon: N-terminal (amino acid position: L7)DepositorInsertgRNA and GFP donor
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Target-AIDmax
Plasmid#139107PurposeMammalian Target-AIDmax expression vector (pSI-626)DepositorInsertBi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sema6d.1-AP-His
Plasmid#72042PurposeExpresses the extracellular region of the Sema6D, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema6d.1 (Sema6d Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX304 SLC2A1
Plasmid#193691PurposeConstitutive lentiviral expression of SLC2A1DepositorInsertSLC2A1 (SLC2A1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-hChR2(H134R)-P2A-EYFP
Plasmid#139283PurposeExpress hChR2(H134R)-P2A-EYFP in a Cre-dependent mannerDepositorInserthChR2(H134R)-P2A-EYFP
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FDIO-COX4-dAPEX2
Plasmid#117178PurposePlasmid name in publication: pAAV-FDIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertCOX4-dAPEX2
UseAAV; Flp/frtTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8f F366H
Plasmid#204141PurposeFluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutationDepositorInsertjGCaMP8f F366H
UseTagsN-terminal His tagExpressionMammalianMutationPhenylalanine 366 changed to histidinePromoterCMVAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-myc3-FBW5 delta F
Plasmid#19912DepositorInsertF-box and WD repeat domain containing 5 delta F (FBXW5 Human)
UseTagsmyc3ExpressionMammalianMutationdeleted aa1-42 (F-box)PromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P shFXN_1
Plasmid#102970PurposeSuppression of FXNDepositorInsertshFXN_1 (FXN Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sema6c-Fc-His
Plasmid#72167PurposeExpresses the extracellular region of the Sema6C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema6c (Sema6c Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe human FOXC2
Plasmid#15535DepositorInsertFOXC2 (FOXC2 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Sst22-GFP
Plasmid#172312PurposeSST interneuron-restricted gene regulatory element GRE22, to drive GFP expression in SST+ interneuronsDepositorInsertSst22
UseAAVTagsExpressionMutationPromoterpB-GlobinAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-vDIO-mScarlet-IRES-tTA
Plasmid#118030PurposeAAV vector for sparse and bright labeling in a cell-type specific mannerDepositorInsertTRE-vDIO-mScarlet-IRES-tTA
UseAAVTagsExpressionMutationPromoterTREAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX459-sgRNA2-RPB1-N-term
Plasmid#195111PurposeVector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).DepositorInsertsgRNA against first exon of RPB1 (N-terminal)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P shFXN_2
Plasmid#102971PurposeSuppression of FXNDepositorInsertshFXN_2 (FXN Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTHMT_hnRNPF_deltaPLD
Plasmid#139115Purposeexpress FL hnRNPF without the prion like domain with N-terminal maltose binding protein tagDepositorInserthnRNPF (HNRNPF Human)
UseTagsExpressionBacterialMutationdelta365-415PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe WT Akt2
Plasmid#14551DepositorInsertAkt2 (AKT2 Human)
UseRetroviralTagsHA tagExpressionMammalianMutationPromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUB-nHyPer2 (NLS:HyPer2:NLS)
Plasmid#84730PurposeExpresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to NucleiDepositorInsertHyPer2 targeted to nucleus
UseTagsNLS (nuclear localisation signal and NLS (nuclear…ExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Flex_syn_SpikeyGi2_kv2.1
Plasmid#195314PurposeGenetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, double-flowed in AAV vector under the promoter hSynDepositorInsertSpikeyGi2
UseAAVTagsExpressionMammalianMutationPromoterhuman synapsin promoterAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hNR2F2-RFP
Plasmid#22926DepositorInserthuman nuclear receptor subfamily 2 group F member 2 promoter driving DsRedExpress (NR2F2 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2
Plasmid#112004PurposeFor enriched expression of GCaMP6s in axons with cytosolic expression of mKate2DepositorHas ServiceAAV9Insertaxon-GCaMP6s-P2A-mKate2
UseAAVTagsGAP43 palmitoylation domainExpressionMammalianMutationPromoterhSynapsin1Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBRPyCAG-hKlf2-DsRed-Ip
Plasmid#26277DepositorInsertKlf2 (KLF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-LACTB-dAPEX2
Plasmid#117180PurposePlasmid name in publication: pAAV-DIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertLACTB-dAPEX2
UseAAV and Cre/LoxTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-fDIO-VARNAM2-Kv2.1PR
Plasmid#195536PurposeFlp recombinase-dependent red fluorescent, negative response-polarity voltage indicator; soma-targetedDepositorInsertVARNAM2-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationVARNAM SI linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Ezr-BioID2-HA
Plasmid#176744PurposeExpresses Ezrin fused with BioID2 at the C-terminus in astrocytesDepositorInsertEzr-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-sgRNA2-2-RPB1-C-term
Plasmid#195109PurposeVector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 C-term. Puromycin selection (2A-fusion).DepositorInsertsgRNA against last exon of RPB1 (C-terminal)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUB-nSypHer (NLS:sypHer:NLS)
Plasmid#84731PurposeExpresses sypHer in plant cells-biosensor for pH targeted to NucleiDepositorInsertnSypHer
UseTagsNLS (nuclear localisation signal and NLS (nuclear…ExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV_hUbC-dSpCas9-2xVP64-T2A-BSD
Plasmid#162333PurposeLentiviral expression of dSpCas9-2xVP64 with blasticidin resistanceDepositorInsertdSpCas9-2xVP64
UseLentiviralTagsFLAGExpressionMammalianMutationD10A and H840APromoterhUbCAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EnhancedSynapsin-DIO-TVA950-EYFP-WPRE
Plasmid#120269PurposeAllows for initial cellular transduction of EnvA-pseudotyped Rb via Cre-dependent expression of an avian TVA receptor fused with yellow fluorescent proteinDepositorInserteSyn-DIO-TVA950:YFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FDIO-LACTB-dAPEX2
Plasmid#117181PurposePlasmid name in publication: pAAV-FDIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertLACTB-dAPEX2
UseAAV; Flp/frtTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5-LAP-MPS1-KD
Plasmid#114045PurposeExpression of exogenous LAP tagged MPS1 Kinase-deadDepositorInsertMPS1-KD (TTK Human)
UseTagsExpressionMammalianMutationD664A kinase deadPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-A'Q-mito
Plasmid#158763PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseTagsExpressionMammalianMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)
Plasmid#47542Purposeengineered receptor improves heteromeric receptor expression and sensitivity to IVM.DepositorInsertGlutamate-gated chloride channels
UseTagsExpressionMammalianMutationretention motif in ER (starting at R340) changed …PromoterCMVAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST- IP2.0(codon for C.elegans)
Plasmid#109216PurposeIP2.0 for C. elegans (Codon optimization and introns).DepositorInsertIP2.0 (Inverse Pericam 2.0)
UseTagsHis-tagExpressionWormMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTACR TH1-p2aGFP
Plasmid#135815PurposeCRISPR based on px330 p2aGFP targeting TH gene near stop codonDepositorInsertTH 1 guide
UseCRISPRTagsp2a Myr-eGFPExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTACR TH2-p2aGFP
Plasmid#135816PurposeCRISPR based on px330 p2aGFP targeting TH gene near stop codonDepositorInsertTH 2 guide
UseCRISPRTagsp2a Myr-eGFPExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-ChETA-TdTomato-WPRE-pA
Plasmid#37755PurposeCre-dependent expression of ChETA-tdTomato, for optogenetic excitationDepositorInsertChETA-TdTomato
UseAAV and Cre/Lox; Cre-onTagsTdTomatoExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.5
Plasmid#111058PurposeExpresses dLight1.5 in mammalian cellsDepositorInsertdLight1.5
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgGAL4-1
Plasmid#46915PurposeHuman pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoterDepositorInsertssgGAL4-1
Puromycin resistance and mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMV and U6Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
mRFP-mem
Plasmid#55622PurposeThis red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mRFP.DepositorInsertmRFP-Mem
UseTagsThe N-terminal 20 residues of neuromodulin are fu…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-FlpOn-CreOff-TdTomato
Plasmid#176275PurposeViral vector for expression of TdTomato in cells expressing Flp AND NOT Cre driven by the human Synapsin promoter.DepositorInserttdTomato
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 JIP3a
Plasmid#53457Purposeinteracts with MAPK signaling moduleDepositorInsertmitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
UseTagsT7ExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPyPGK-HA-EGFP-Myc-TM-IRES-Pac
Plasmid#183606PurposeMammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.DepositorInsertEGFP-TM
UseTagsHA, Human PDGFRB transmembrane domain, Mouse IgGK…ExpressionMammalianMutationPromoterMouse Pgk1Available sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHDR-eGFP (pXK-1647)
Plasmid#208824PurposeThe HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDRDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLIC-His-RGS4
Plasmid#204847PurposeExpress His tagged-RGS4 in bacterial cells for protein purificationDepositorInsertRGS4 (RGS4 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-GluA1
Plasmid#173008PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.DepositorInsertDHFR-GFP-th-GluA1
UseLentiviralTagsGFPExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.Twitch2B.WPRE.SV40
Plasmid#100039PurposeAAV expression of FLEXed Twitch2B driven by CAG promoter. Twitch 2B is a fluorescent reporter for calcium signalingDepositorInsertTwitch2B
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sema3e(L)-Fc-His
Plasmid#72145PurposeExpresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3e (Sema3e Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntn1-AP-His
Plasmid#71978PurposeExpresses the entire Netrin 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNtn1 (Ntn1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2016AvailabilityAcademic Institutions and Nonprofits only