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Showing: 4501 - 4520 of 6514 results

  4. pYUBcLIC-GFP

    Plasmid
    #183509
    Purpose
    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.
    Depositor
    Martin Högbom
    Article
    Grave et al Protein Expr Purif. 2022 Oct;198:106132.
    Type
    Empty backbone
    Use
    Tags
    10x His-tag and folding reporter GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    183509-plasmid-map-sequence-id-362497

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  9. pQE80l BCB

    Plasmid
    #196472
    Purpose
    Protein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher
    Depositor
    Fei Sun
    Article
    Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114
    Insert
    BCB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196472-plasmid-map-sequence-id-387266

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  11. FnR8-10

    Plasmid
    #182569
    Purpose
    Human fibronectin fragment, type-III repeats 8-10, R1379A mutant
    Depositor
    Timothy Springer
    Article
    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi
    Insert
    Human fibronectin fragment, type-III repeats 8-10
    Use
    Tags
    His and TEV cleavage site after His tag
    Expression
    Bacterial
    Mutation
    R1379A
    Promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182569-plasmid-map-sequence-id-357183

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  14. pBEL2265

    Plasmid
    #175962
    Purpose
    For expresson of LetA-[LetB PLL3->6] operon from E. coli. N-terminal his tag on LetA, araBAD promoter
    Depositor
    Damian Ekiert, Gira Bhabha
    Article
    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d
    Inserts
    LetA
    LetB
    Use
    Tags
    8xHis 2xQH and TEV cleavage site on LetA
    Expression
    Bacterial
    Mutation
    Δ347-361, replace with 702-716
    Promoter
    araBAD
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    175962-plasmid-map-sequence-id-345244

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  17. pET28a(+)-HisTim12_CtoA

    Plasmid
    #170281
    Purpose
    Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production
    Depositor
    Paul Schanda
    Article
    Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2
    Insert
    yeast Tim12 (TIM12 Budding Yeast)
    Use
    Tags
    TEV-cleavable N-terminal His tag (on backbone)
    Expression
    Bacterial
    Mutation
    mutated two non-conserved Cys to Ala
    Promoter
    Available since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170281-plasmid-map-sequence-id-334955

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  20. pGBW-m4136457

    Plasmid
    #149537
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149537-plasmid-map-sequence-id-314686

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Showing: 4501 - 4520 of 6514 results