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Plasmid#128614PurposeMammalian Expression Plasmid of anti-Kv1.2 K+ channel (Human). Derived from hybridoma K14/16.DepositorInsertanti-Kv1.2 K+ Channel (Human) recombinant mouse monoclonal antibody (KCNA2 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-mCherry-IRESMygro
Plasmid#82505PurposeVector targeting the mCherry gene to the GAPDH locus of human cells. mCherry is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.DepositorInsertmCherry-IRESMygro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-Yap S127A
Plasmid#46050DepositorInsertYes-kinase associated protein (YAP1 Human)
UseEntry vectorTags2X Flag tagExpressionMutationS127APromoterAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pGAPTrap-IRESMuro
Plasmid#82336PurposeBase vector targeting genes to the GAPDH locus of human cells. Inserted genes are expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertIRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX304-PCDH7-c
Plasmid#108540PurposeExpresses human PCDH7 transcript variant c in mammalian cellsDepositorInsertPCDH7 transcript variant c (PCDH7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX304-PCDH7-b
Plasmid#108539PurposeExpresses human PCDH7 transcript variant b in mammalian cellsDepositorInsertPCDH7 transcript variant b (PCDH7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pGAPTrap-mtagtdTom_IRESMuro
Plasmid#82355PurposeVector targeting the mTagtandem tomato gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertmtagTandemTomato-IRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKK-BI16-ORF1-3C-FLAG_ORF2-TEV-mClover3
Plasmid#105803PurposeConcomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C protease; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.DepositorTypeEmpty backboneUseFlp-in competentTagsORF1: 3C-FLAG; ORF2: TEV-mClover3ExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-eGFP-IRESMuro
Plasmid#82504PurposeVector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInserteGFP-IRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-Luc2-IRESMeo
Plasmid#82509PurposeVector targeting the Firefly Luciferase (Luc2) gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Meo gene.DepositorInsertLuc2-IRESMeo
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-kappa-HA-dL5-myc-ADRB2
Plasmid#101253PurposeExpresses HA-dL5(E52D)-cMyc N-terminal fusion of beta-2 adrenergic receptor in mammalian cells, MMLV retroviral plasmid (MBIC5, dL5**, FAP, ADRB2)DepositorInsertkappa-HA-dL5-myc-ADRB2 (ADRB2 Human, Budding Yeast)
UseRetroviralTagsFAP-dL5 and HA epitopeExpressionMammalianMutationPromoterMMLV LTRAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-eGFP-IRESMygro
Plasmid#82503PurposeVector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.DepositorInserteGFP-IRESMygro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pet-ECHB(35-475)
Plasmid#96890Purposeexpresses His-tagged mouse ECHB (35-475) in E.coli cellsDepositorInsertTrifunctional enzyme subunit beta, mitochondrial (Hadhb Mouse)
UseTagsHis6 tagExpressionBacterialMutationdeleted amino acid 1-34PromoterT7Available sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet-ECHA(37-763)-FLAG (no His-tag)
Plasmid#96887Purposeexpresses flag-tagged mouse ECHA (37-768) in E.coli cells (no His-tag)DepositorInsertTrifunctional enzyme subunit alpha, mitochondrial (Hadha Mouse)
UseTagsFLAG-taggedExpressionBacterialMutationdeleted amino acid 1-36PromoterT7Available sinceJuly 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber
Plasmid#105806PurposeConcomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.DepositorTypeEmpty backboneUseFlp-in competentTagsORF1: 3C-Cerulean; ORF2: TEV-AmberExpressionMammalianMutationPromoterAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Anti-KCNU1/Slo3 maxi-K+ channel [N2/16R]
Plasmid#114541PurposeMammalian Expression Plasmid of anti-KCNU1/Slo3 maxi-K+ channel (Mouse). Derived from hybridoma N2/16.DepositorInsertanti-KCNU1/Slo3 maxi-K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnu1 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-LacZ-IRESMuro
Plasmid#82507PurposeVector targeting the LacZ gene to the GAPDH locus of human cells. LacZ is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertLacZ-IRESMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 NDUFS6(5’UTR)-FF
Plasmid#85488PurposeFirefly luciferase under the control of NDUFS6 5'UTRDepositorInsert5'UTR of NDUFS6 (NDUFS6 Human)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only