High Complexity Golden Gate Assembly with NEB’s DAD
Type
Blog Post
...
37-16 °C
cycling
4-base
Bsal-HFv2
37-16 °C
cycling
4-base
BsmBI-v2
42-16 °C
cycling...NEBridge Ligase MM
42-16 °C
cycling
4-base
BspQI, 1x NEBridge Ligase MM
42-16 °C
cycling
3-base...Ligase Buffer
37-16 °C
cycling
4-base
PaqCl, 1x NEBridge Ligase MM
37-16 °C
cycling
4-base...
37-16 °C
cycling
3-base
Sapl, 1x NEBridge Ligase MM
37-16 °C
cycling
3-base... 4-base
Bbsl-HF®, 1x NEBridge® Ligase MM
37-16 °C
cycling
4-base
Bsal-HFv2®
37 °C
Static...