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Showing: 1 - 20 of 103 results
  1. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ... 37-16 °C cycling 4-base Bsal-HFv2 37-16 °C cycling 4-base BsmBI-v2 42-16 °C cycling...NEBridge Ligase MM 42-16 °C cycling 4-base BspQI, 1x NEBridge Ligase MM 42-16 °C cycling 3-base...Ligase Buffer 37-16 °C cycling 4-base PaqCl, 1x NEBridge Ligase MM 37-16 °C cycling 4-base...              37-16 °C cycling 3-base Sapl, 1x NEBridge Ligase MM 37-16 °C cycling 3-base... 4-base Bbsl-HF®, 1x NEBridge® Ligase MM 37-16 °C cycling 4-base Bsal-HFv2® 37 °C Static...
  2. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...fontawesome-5.14.0" name="Check" style="SOLID" height="16" purpose="decorative" title="Check icon" %} Just ...fontawesome-5.14.0" name="Check" style="SOLID" height="16" purpose="decorative" title="Check icon" %} Satellite...fontawesome-5.14.0" name="Check" style="SOLID" height="16" purpose="decorative" title="Check icon" %} {% icon...fontawesome-5.14.0" name="Check" style="SOLID" height="16" purpose="decorative" title="Check icon" %} Carbenicillin...fontawesome-5.14.0" name="Check" style="SOLID" height="16" purpose="decorative" title="Check icon" %} Spectinomycin...
  3. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ... plasmids called TRUPATH to interrogate 14 of the 16 non-visual G protein signal transducers (collectively...generate an optimal Rluc8-Gα construct for two of the 16 non-visual G protein transducers. To use the TRUPATH...interrogating the GPCR transducerome. Nat Chem Biol 16:841–849 . https://doi.org/10.1038/s41589-020-0535...
  4. TALENs for the EGFP Reporter Gene

    Type
    Collection
    ...TAL2029 TGCGCTCCTGGAC eGFP-TALEN-16-Left TAL2030 TCAAGGAGGACGG eGFP-TALEN-16-Right TAL2031 TGTACTCCAGCTT eGFP-TALEN...
  5. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...each with a different complement strand covering all 16 possible NN pairs in the template. The probes were...Watson-Crick and mismatched) around the ligation junction in 16 wells of a 96-well plate, allowing 6 conditions to...reaction in a PCR world." PCR Methods Appl 1.1 (1991): 5-16. PubMed PMID: 1842922. 13. Wiedmann, M., et al. "...blood-borne bacterial pathogens by use of a novel 16S rRNA gene PCR-ligase detection reaction-capillary...PMID: 17428930. PubMed Central PMCID: PMC1933048. 16. Cheng, Changming, et al. "Electrochemiluminescent...
  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ...diversity 3-10 DXP'1, IGHD310 IGHD3-16 immunoglobulin heavy diversity 3-16 IGHD316 IGHD3-22 immunoglobulin...variable 3-15 IGHV315, VH IGHV3-16 immunoglobulin heavy variable 3-16 (non-functional) IGHV316, VH IGHV3.../pseudogene) IGKV113, L18 IGKV1-16 immunoglobulin kappa variable 1-16 IGKV116, L1 IGKV1-17 immunoglobulin...variable 1D-13 IGKV1D13, L18 IGKV1D-16 immunoglobulin kappa variable 1D-16 IGKV1D16, L15, L15a IGKV1D-17 immunoglobulin...variable 3-12 IGLV312, V2-8 IGLV3-16 immunoglobulin lambda variable 3-16 IGLV316, V2-11 IGLV3-19 immunoglobulin...interleukin 16 (lymphocyte chemoattractant factor) FLJ16806, FLJ42735, FLJ44234, HsT19289, IL-16, LCF, prIL...
  7. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...often use next-generation sequencing. Sequencing the 16S rRNA reveals the identity of the organisms present...the “trigger RNA” (in this case, biomarker RNA or 16S rRNA isolated from the microbial community) reveals...
  8. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...ultrastructures using expansion microscopy (U-ExM). Nat Methods 16:71–74. https://doi.org/10.1038/s41592-018-0238-1  ...principles and uses in biological research. Nat Methods 16:33–41. https://doi.org/10.1038/s41592-018-0219-4  ...
Showing: 1 - 20 of 103 results