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Showing: 1 - 20 of 79 results
  1. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ... 37-16 °C cycling 4-base Bsal-HFv2 37-16 °C cycling 4-base BsmBI-v2 42-16 °C cycling...NEBridge Ligase MM 42-16 °C cycling 4-base BspQI, 1x NEBridge Ligase MM 42-16 °C cycling 3-base...Ligase Buffer 37-16 °C cycling 4-base PaqCl, 1x NEBridge Ligase MM 37-16 °C cycling 4-base...              37-16 °C cycling 3-base Sapl, 1x NEBridge Ligase MM 37-16 °C cycling 3-base... 4-base Bbsl-HF®, 1x NEBridge® Ligase MM 37-16 °C cycling 4-base Bsal-HFv2® 37 °C Static...
  2. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...fontawesome-5.14.0" name="Check" style="SOLID" height="16" purpose="decorative" title="Check icon" %} Just ...fontawesome-5.14.0" name="Check" style="SOLID" height="16" purpose="decorative" title="Check icon" %} Satellite...fontawesome-5.14.0" name="Check" style="SOLID" height="16" purpose="decorative" title="Check icon" %} {% icon...fontawesome-5.14.0" name="Check" style="SOLID" height="16" purpose="decorative" title="Check icon" %} Carbenicillin...fontawesome-5.14.0" name="Check" style="SOLID" height="16" purpose="decorative" title="Check icon" %} Spectinomycin...
  3. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ...laboratory cultures to study these microbes. From 16S rRNA sequencing, hot springs were known to be diversity...Potanina) Neddersen characterized a few samples by 16S rRNA gene sequencing which showed the presence of...publication is the removal of unwanted cDNA derived from 16S rRNA in bacterial RNA-Seq. This enriches for transcripts...
  4. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ... plasmids called TRUPATH to interrogate 14 of the 16 non-visual G protein signal transducers (collectively...generate an optimal Rluc8-Gα construct for two of the 16 non-visual G protein transducers. To use the TRUPATH...interrogating the GPCR transducerome. Nat Chem Biol 16:841–849 . https://doi.org/10.1038/s41589-020-0535...
  5. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...each with a different complement strand covering all 16 possible NN pairs in the template. The probes were...Watson-Crick and mismatched) around the ligation junction in 16 wells of a 96-well plate, allowing 6 conditions to...reaction in a PCR world." PCR Methods Appl 1.1 (1991): 5-16. PubMed PMID: 1842922. 13. Wiedmann, M., et al. "...blood-borne bacterial pathogens by use of a novel 16S rRNA gene PCR-ligase detection reaction-capillary...PMID: 17428930. PubMed Central PMCID: PMC1933048. 16. Cheng, Changming, et al. "Electrochemiluminescent...
  6. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...often use next-generation sequencing. Sequencing the 16S rRNA reveals the identity of the organisms present...the “trigger RNA” (in this case, biomarker RNA or 16S rRNA isolated from the microbial community) reveals...
  7. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...(4.5) 7.2 (5.2) xCas9(3.7)-BE3 37 (1.3) 16 (3.5) 6.2 (13) 24 (9.5) 10 (>50) 12 (>100...ABE 69 (1.4) 43 (2) 21 (3) Not examined 16 (>100) Average percent editing efficiencies (...
  8. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...ultrastructures using expansion microscopy (U-ExM). Nat Methods 16:71–74. https://doi.org/10.1038/s41592-018-0238-1  ...principles and uses in biological research. Nat Methods 16:33–41. https://doi.org/10.1038/s41592-018-0219-4  ...
Showing: 1 - 20 of 79 results