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Plasmid#223138PurposeAAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffoldDepositorTypeEmpty backboneUseAAV and CRISPRTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only
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pBK1290-AAV-dSaCas9
Plasmid#223139PurposeAAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA ScaffoldDepositorTypeEmpty backboneUseAAV and CRISPRTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn RSET-T-GECO1.WPRE
Plasmid#211902PurposeExpresses T-GECO1 in mammalian cellsDepositorInsertT-GECO1
UseAAVTagsRSET peptideExpressionMammalianMutationPromoterhSynAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-IvfChr-citrine-KV2.1
Plasmid#221615PurposeA recombinant AAV2 plasmid encoding vfChrimson-citrine with trafficking enhancement and soma targeting with KV2.1 motif.DepositorInsertvfChrimson-citrine with membrane trafficking enhancement and soma targeting
UseAAVTagsExpressionMutationvfChrimson-citrine with membrane trafficking enha…PromoterHuman SynapsinAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF1b-His8-TEV-PI31-1-151
Plasmid#202593PurposeBacterial expression of HsPI31-1-151DepositorInsertPI31 (PSMF1 Human)
UseTags8xHis, TEVExpressionBacterialMutationaa 1-151 onlyPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK6031
Plasmid#219739PurposeMoClo-compatible Level 0 - promoter + 5U vector carrying promoter CmYLCVDepositorInsertCestrum yellow leaf curling virus (CmYLCV) promoter
UseSynthetic BiologyTagsExpressionPlantMutationPromoterCmYLCVAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-EGFR-L858R-tmpknot-epegRNA
Plasmid#214092PurposeLentiviral vector expressing epegRNA to induce EGFR L858R mutationDepositorInsertEGFR L858R epegRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-EGFR-T790M-tmpknot-epegRNA
Plasmid#214093PurposeLentiviral vector expressing epegRNA to induce EGFR T790M mutationDepositorInsertEGFR T790M epegRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-KRAS-G12C-tmpknot-epegRNA
Plasmid#214094PurposeLentiviral vector expressing epegRNA to induce KRAS G12C mutationDepositorInsertKRAS G12C epegRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-KRAS-G13A-tmpknot-epegRNA
Plasmid#214095PurposeLentiviral vector expressing epegRNA to induce KRAS G13A mutationDepositorInsertKRAS G13A epegRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA333
Plasmid#216067PurposeFragmid fragment: (2A Selection) Stop; IRES2; Start; PuroR; StopDepositorInsertStop; IRES2_v1; Start; PuroR_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
ULK1-Halo HRD
Plasmid#207548PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cellsDepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-BRX-eGFP-NLS
Plasmid#217534PurposeAAV vector to drive the expression of eGFP under the control of the 4xBRE regulatory element of SMAD1 and miniXon splicing casette transcription factor in vivoDepositorArticleInsertBMP reporter element and miniXon casette (Smad1 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterBMP Reporter ElementAvailable sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP
Plasmid#179717PurposeLentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFPDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2 RPS28 UTR1 SCR
Plasmid#203158PurposeRPS28 UTR luciferase assay plasmid (SCR UTR1, sites 1 and 2)DepositorInsertRPS28 UTR (RPS28 Human)
UseLuciferaseTagsExpressionMutationscrambled the nucleotides in the two MIR-28 bindi…PromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Sik-WT
Plasmid#210722PurposeConstruct for stable expression of mouse wild type Ptk6 (Sik) in mammalian cells.DepositorInsertProtein tyrosine kinase 6 (Ptk6 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Sik-YF
Plasmid#210723PurposeConstruct for stable expression of constitutively active mouse Ptk6 (Sik) in mammalian cells.DepositorInsertProtein tyrosine kinase 6 (Ptk6 Mouse)
UseTagsExpressionMammalianMutationTyrosine 447 changed to PhenylalaninePromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC150-KEIMA-REEP5
Plasmid#201928PurposePiggyBac Vector expressing KEIMA-REEP5DepositorInsertREEP5 (REEP5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PTK6-YF
Plasmid#210715PurposeConstruct for stable expression of constitutively active human PTK6 in mammalian cells.DepositorInsertProtein tyrosine kinase 6 (PTK6 Human)
UseTagsExpressionMammalianMutationTyrosine 447 changed to PhenylalaninePromoterAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Combined γB2FL-FRET probe
Plasmid#209094PurposemTurquoise2- and mVenus-inserted PcdhgB2 full length (FL)DepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsPA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Combined γB2ΔICD-FRET probe
Plasmid#209093PurposemTurquoise2- and mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)DepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsPA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSSA-eGFP (pXK-221)
Plasmid#208826PurposeThe SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH50 - [PVQp | gfp | tbb-2 UTR]
Plasmid#200326Purposegfp BioPart for PVQ neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[PVQp | gfp | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00003755, nlp-17Available sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF3C CC 411-615
Plasmid#193936PurposeMammalian expression of Halo-tagged Kif 3C CC 411-615DepositorInsertKif 3C (Kif3c Mouse)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceSept. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP barcode-2-SV40 polyA
Plasmid#190866PurposeFor barcoded retrograde labelingDepositorInsertEGFP-barcode2
UseAAVTagsExpressionMutationWTPromoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-GUS
Plasmid#202137PurposeLevel 0 plasmid containing a beta glucuronidase reporter gene with CD overhangs used to build a level 1 construct.DepositorInsertBeta glucuronidase gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CAG-EGFPnls barcode-210-SV40 polyA
Plasmid#190876PurposeFor barcoded retrograde labelingDepositorInsertEGFPnls-barcode210
UseAAVTagsExpressionMutationWTPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF3A CC 419-589
Plasmid#193926PurposeMammalian expression of Halo-tagged Kif 3A CC 419-589DepositorInsertKif 3A (KIF3A Human)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP barcode-9-SV40 polyA
Plasmid#190873PurposeFor barcoded retrograde labelingDepositorInsertEGFP-barcode9
UseAAVTagsExpressionMutationWTPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-MD-mTurquoise
Plasmid#193094Purposeconstitutive expression of the mitosis degradation (MD) domain of cyclin B1 fused to mTurquoise in mammalian cellsDepositorInsertMD-mTurquoise
UseLentiviralTagsExpressionMammalianMutationPromoterUbcAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYT1deltaC2AB reporter
Plasmid#197279PurposeEncodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 a.a.) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYT1 (1 - 420 a.a.) and HaloTag (Syt1 Rat)
UseLentiviralTagsFLAGExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/sh-FYN1-GFP_Seq_C
Plasmid#201399PurposeKnockdown of FYN tyrosine kinase. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertFYN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3B DD 593-747
Plasmid#193932PurposeMammalian expression of GFP-tagged Kif 3B DD 593-747DepositorInsertKif 3B (Kif3b Mouse)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR015
Plasmid#198812PurposeEpidermal growth factorDepositorInsert15xUAS::Lin-3c-SL2-GFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR026
Plasmid#198824PurposeLysosomal associated membrane proteinDepositorInsert15xUAS::lmp1-venus::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW450
Plasmid#198831PurposeVoltage-sensitive fluorescent protein.DepositorInsert15xUAS::VSFP-Butterfly-1-2::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 gfaABC1D-eGFP-SAPAP3
Plasmid#176859PurposeExpresses SAPAP3 protein fused with eGFP at the N-terminus in astrocytesDepositorInserteGFP-SAPAP3
UseAAVTagsGFPExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2ss-U6-sgGFP
Plasmid#190899PurposeAAV vector expressing sgGFPDepositorInsertsgRNA
UseAAV and CRISPRTagsExpressionMutationPromoterU6Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-alphaCaMKII-dsRed-Express-3XNLS-pA
Plasmid#183802PurposeAAV vector to express nuclear localized dsRed-Express from CaMKIIa promoterDepositorInsertdsRed-Express
UseAAVTagsNLSExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
etnppl-pGEMtEasy
Plasmid#192263PurposeMouse Etnppl sequence is inserted in pGEM-T Easy vector.DepositorInsertethanolamine phosphate phospholyase (Etnppl Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(ETNPPL)
Plasmid#192264PurposeCoding sequence of mouse ETNPPL is inserted in pET-15b vectorDepositorInsertethanolamine phosphate phospholyase (Etnppl Mouse)
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miniSOG-C-Far
Plasmid#193914PurposeControl plasmid that expresses miniSOG targeted to the cell membrane in mammalian cellsDepositorInsertminiSOG
UseAAVTagsFarnesylation signal sequence and Flag tagExpressionMammalianMutationPromoterCAGAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-IC2N-dCer3
Plasmid#191325PurposeChronically inhibit dynein by expressing the dominant negative acting N-terminal domain of the dynein intermediate chain (IC2N)DepositorInsertIC2N-dCer-FRB (Dync1i2 Mouse)
UseTagsExpressionMammalianMutationaa 1-237PromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF3C tail 411-796
Plasmid#193934PurposeMammalian expression of Halo-tagged Kif 3C tail 411-796DepositorInsertKif 3C (Kif3c Mouse)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF3C DD 616-796
Plasmid#193938PurposeMammalian expression of Halo-tagged Kif 3C DD 616-796DepositorInsertKif 3C (Kif3c Mouse)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3C DD 616-796
Plasmid#193937PurposeMammalian expression of GFP-tagged Kif 3C DD 616-796DepositorInsertKif 3C (Kif3c Mouse)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KAP3B
Plasmid#193940PurposeMammalian expression of Halo-tagged KAP 3BDepositorInsertKAP 3B (KIFAP3 Human)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3B CC 391-592
Plasmid#193930PurposeMammalian expression of GFP-tagged Kif 3B CC 391-592DepositorInsertKif 3B (Kif3b Mouse)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3C CC 411-615
Plasmid#193935PurposeMammalian expression of GFP-tagged Kif 3C CC 411-615DepositorInsertKif 3C (Kif3c Mouse)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF3B CC 391-592
Plasmid#193931PurposeMammalian expression of Halo-tagged Kif 3B CC 391-592DepositorInsertKif 3B (Kif3b Mouse)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only