Skip to main content
Addgene
Showing: 4341 - 4360 of 5316 results

  1. pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113698
    Purpose
    Dox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113698-plasmid-map-sequence-id-232033

    Enlarge

  2. pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR

    Plasmid
    #192939
    Purpose
    FlpO-based digitizer circuit driven by AP1 promoter
    Depositor
    Wilson Wong
    Article
    Wong et al Nat Commun. 2021 Oct 22;12(1):6150. doi:
    Inserts
    AP1(2)/minTK promoter
    FlpO-ABI
    PYL-FlpO
    CAG promoter
    frt-STOP-frt
    GFP
    luciferase
    pPGK
    Zeocin resistance
    BFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    192939-plasmid-map-sequence-id-380399

    Enlarge

  3. pAdDeltaF6

    Plasmid
    #112867
    Purpose
    AAV helper plasmid
    Depositor
    James M. Wilson
    Insert
    E4, E2a and VA
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    112867-plasmid-map-sequence-id-372973

    Enlarge

  4. pZac2.1 gfaABC1D-cyto-GCaMP8s

    Plasmid
    #186052
    Purpose
    AAV-mediated expression of GCaMP8s under the control of GfaABC1D promoter
    Depositor
    Loren Looger
    Insert
    jGCaMP8s
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    GfaABC1D
    Available since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186052-plasmid-map-sequence-id-370168

    Enlarge

  5. pZac2.1 gfaABC1D-cyto-GCaMP8f

    Plasmid
    #186050
    Purpose
    AAV-mediated expression of GCaMP8f under the control of GfaABC1D promoter
    Depositor
    Loren Looger
    Insert
    jGCaMP8f
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    GfaABC1D
    Available since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186050-plasmid-map-sequence-id-370166

    Enlarge

  6. pZac2.1 gfaABC1D-cyto-GCaMP8m

    Plasmid
    #186051
    Purpose
    AAV-mediated expression of GCaMP8m under the control of GfaABC1D promoter
    Depositor
    Loren Looger
    Insert
    jGCaMP8m
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    GfaABC1D
    Available since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186051-plasmid-map-sequence-id-370165

    Enlarge

  7. Syn-MCS-WPRE-SV40 - GCH7

    Plasmid
    #230817
    Purpose
    AAV cloning vector for expression under the Syn promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230817-plasmid-map-sequence-id-457138

    Enlarge

  8. CaMKII-MCS-WPRE-SV40 - GCH19

    Plasmid
    #230823
    Purpose
    AAV cloning vector for expression under the CaMKII promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230823-plasmid-map-sequence-id-457140

    Enlarge

  9. EF1a-MCS-WPRE-SV40 - GCH13

    Plasmid
    #230820
    Purpose
    AAV cloning vector for expression under the EF1a promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230820-plasmid-map-sequence-id-457137

    Enlarge

  10. GFAP-MCS-WPRE-SV40 - GCH31

    Plasmid
    #230829
    Purpose
    AAV cloning vector for expression under the GFAP promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230829-plasmid-map-sequence-id-457134

    Enlarge

  11. CaMKII-FRT-MCS-WPRE-SV40 - GCH21

    Plasmid
    #230825
    Purpose
    AAV cloning vector for Flpo dependent expression under the CaMKII promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230825-plasmid-map-sequence-id-457144

    Enlarge

  12. EF1a-FRT-MCS-WPRE-SV40 - GCH15

    Plasmid
    #230822
    Purpose
    AAV cloning vector for Flpo dependent expression under the EF1a promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230822-plasmid-map-sequence-id-457143

    Enlarge

  13. GFAP-FRT-MCS-WPRE-SV40 - GCH33

    Plasmid
    #230831
    Purpose
    AAV cloning vector for Flpo dependent expression under the GFAP promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230831-plasmid-map-sequence-id-457141

    Enlarge

  14. EF1a-Flex-MCS-WPRE-SV40 - GCH14

    Plasmid
    #230821
    Purpose
    AAV cloning vector for CRE dependent expression under the EF1a promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230821-plasmid-map-sequence-id-457139

    Enlarge

  15. Syn-FLEX-MCS-WPRE-SV40 - GCH8

    Plasmid
    #230818
    Purpose
    AAV cloning vector for CRE dependent expression under the Syn promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230818-plasmid-map-sequence-id-457133

    Enlarge

  16. mini-pHelper-1.0

    Plasmid
    #230933
    Purpose
    The mini verison (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production
    Depositor
    Qizhao Wang
    Type
    Empty backbone
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230933-plasmid-map-sequence-id-458355

    Enlarge

Showing: 4341 - 4360 of 5316 results