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SNAP-Sec61B

Plasmid

#141152

Purpose

Fluorescent labeling of ER membrane in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Sec61 beta (SEC61B Human)

Use

Tags

SNAP tag

Expression

Mammalian

Mutation

L79M on Sec61B

Promoter

CMV

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_AAVS1_T2

Plasmid

#79888

Purpose

Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR

Tags

Untagged eSpCas9(1.1)

Expression

Mammalian

Mutation

Promoter

CBh

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-UBF

Plasmid

#17656

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

UBF (UBTF Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2008

Availability

Academic Institutions and Nonprofits only

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lenti U6-sgRNA/EF1a-mCherry

Plasmid

#114199

Purpose

Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

sgRNA scaffold

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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PB-LacI-eGFP

Plasmid

#213021

Purpose

piggyBac donor vector containing LacI-eGFP for random integration by PBase

Depositor

Luca Giorgetti

Article

Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

Insert

LacI**-eGFP

Use

Mouse Targeting; Piggybase integration (transposo…

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pSB-TRE-CRISPRoff-EF1A-TetOn

Plasmid

#203355

Purpose

Drug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backbone

Depositor

Jonathan Brunger

Article

Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

Insert

CRISPRoff-TetOn

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pmGFP-ADAR1-p150

Plasmid

#117927

Purpose

ADAR1-p150 expression plasmid.

Depositor

Kumiko Ui-Tei

Article

Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

Insert

ADAR1-p150 (ADAR Human)

Use

Tags

myc-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pYFJ16-LplA(W37V) (for E. Coli expression)

Plasmid

#34838

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LplA W37V (lplA E. Coli)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Mutated Trp37 to Val

Promoter

T5 promoter

Available since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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EJ2GFP-puro

Plasmid

#44025

Depositor

Jeremy Stark

Article

Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

Insert

EJ2GFP egfp-based chromosomal break reporter

Use

Tags

Expression

Mammalian

Mutation

insertion of I-SceI, 3 frame stop, and flanking m…

Promoter

pCAGGS

Available since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-GDH

Plasmid

#108954

Purpose

heterologous expression of GDH of B. megaterium in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Glucose dehydrogenase IV (GDH IV)

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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PARP1 FL

Plasmid

#169815

Purpose

Expresses codon optimised full length His tagged PARP1 in bacterial cells

Depositor

David Neuhaus

Article

Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

Insert

Poly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Synthetic, Human)

Use

Tags

MKHHHHHHMKQ

Expression

Bacterial

Mutation

Valine 762 mutated to an alanine

Promoter

T7 promoter

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3a WT

Plasmid

#1787

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a (FOXO3 Human)

Use

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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p6351 MSCV-CMV-CMV-Flag-HA-NSD3

Plasmid

#31357

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

NSD3 (NSD3 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2011

Availability

Academic Institutions and Nonprofits only

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FUGW-Mito-TurboID

Plasmid

#200963

Purpose

3rd gen lentiviral plasmid for expressing mitochondria localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Mito-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG RpH-LAMP1-3xFLAG

Plasmid

#163018

Purpose

expresses ratiometric sensor of lysosomal lumenal pH

Depositor

Massimiliano Stagi

Article

Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

Insert

RpH-LAMP1-3xFLAG (Lamp1 Mouse)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-AML1/ETO-IRES-GFP

Plasmid

#60832

Purpose

AML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFP

Depositor

Scott Lowe

Article

Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

Insert

AML1/ETO (RUNX1, RUNX1T1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pDRM56 tet-AR(1-707)C617Y

Plasmid

#183504

Purpose

Lentiviral vector expressing a doxycycline-inducible truncated DNA-binding mutant androgen receptor (AR mutant C617Y)

Depositor

Wayne Tilley

Article

Hickey et al Nat Med. 2021 Feb;27(2):310-320. doi: 10

Insert

Androgen receptor (AR Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Truncated mutant AR; spans AR 1-707 aa, and conta…

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag ERbeta

Plasmid

#35562

Depositor

Harish Srinivas

Article

Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

Insert

Estrogen Receptor beta (ESR2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2012

Availability

Academic Institutions and Nonprofits only

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FUGW

Plasmid

#14883

Purpose

3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for cDNA expresion

Depositor

David Baltimore

Article

Lois et al Science. 2002 Feb 1. 295(5556):868-72.

Insert

flap-Ub promoter-GFP-WRE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2007

Availability

Academic Institutions and Nonprofits only

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pMK1254

Plasmid

#133057

Purpose

Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mRuby2 fusion protein.

Depositor

Martin Kampmann

Article

Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

Insert

Tau.K18(LM)

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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