pBUN250
(Plasmid
#60672)
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Purposeoverexpression of M. smegmatis ddl gene in Mycobacterium
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 60672 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMV262
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Backbone manufacturerMedImmune Inc.
- Total vector size (bp) 5700
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Vector typeE.coli-Mycobacterium shuttle vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameddl
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SpeciesM. smegmatis
- Promoter BCG Hsp60
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBUN250 was a gift from Raul Barletta (Addgene plasmid # 60672 ; http://n2t.net/addgene:60672 ; RRID:Addgene_60672) -
For your References section:
Roles of Mycobacterium smegmatis D-alanine:D-alanine ligase and D-alanine racemase in the mechanisms of action of and resistance to the peptidoglycan inhibitor D-cycloserine. Feng Z, Barletta RG. Antimicrob Agents Chemother. 2003 Jan;47(1):283-91. PubMed 12499203
Map uploaded by the depositor.