pBUN279
(Plasmid
#60674)
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Purposeintegration of M. smegmatis ald gene in Mycobacterium using mycobacteriophage L5 attB site
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 60674 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYUB412
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Backbone manufacturerPavelka and Jacobs, PMID 10438745
- Total vector size (bp) 10100
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Vector typeE.coli-Mycobacterium integration-proficient vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameald
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Alt nameL-Alanine Dehydrogenase
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SpeciesM. smegmatis
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GenBank IDAF304867
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBUN279 was a gift from Raul Barletta (Addgene plasmid # 60674 ; http://n2t.net/addgene:60674 ; RRID:Addgene_60674) -
For your References section:
Mycobacterium smegmatis L-alanine dehydrogenase (Ald) is required for proficient utilization of alanine as a sole nitrogen source and sustained anaerobic growth. Feng Z, Caceres NE, Sarath G, Barletta RG. J Bacteriol. 2002 Sep;184(18):5001-10. PubMed 12193615
Map uploaded by the depositor.