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Addgene

pBUN279
(Plasmid #60674)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60674 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pYUB412
  • Backbone manufacturer
    Pavelka and Jacobs, PMID 10438745
  • Total vector size (bp) 10100
  • Vector type
    E.coli-Mycobacterium integration-proficient vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ald
  • Alt name
    L-Alanine Dehydrogenase
  • Species
    M. smegmatis
  • GenBank ID
    AF304867

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBUN279 was a gift from Raul Barletta (Addgene plasmid # 60674 ; http://n2t.net/addgene:60674 ; RRID:Addgene_60674)
  • For your References section:

    Mycobacterium smegmatis L-alanine dehydrogenase (Ald) is required for proficient utilization of alanine as a sole nitrogen source and sustained anaerobic growth. Feng Z, Caceres NE, Sarath G, Barletta RG. J Bacteriol. 2002 Sep;184(18):5001-10. PubMed 12193615