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Showing: 10551 - 10600 of 28572 results

  1. pHDR-USR-eGFP

    Plasmid
    #208830
    Purpose
    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed eGFP but without Cas9. The selective PuroR reporter gene was designed to be repaired only by the sgRNA/Cas9-triggered HDR.
    Depositor
    Zhiying Zhang
    Article
    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, U6
    Available since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    208830-plasmid-map-sequence-id-414896

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  6. pFsynW SYB2 reporter

    Plasmid
    #197276
    Purpose
    Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYB2 and HaloTag (Vamp2 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    N/A
    Promoter
    hSyn (human synapsin I promoter)
    Available since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197276-plasmid-map-sequence-id-389092

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  11. pL-SFFV.Reporter.RFP657.PAC

    Plasmid
    #61395
    Purpose
    Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.
    Depositor
    Benjamin Ebert
    Article
    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    61395-plasmid-map-sequence-id-101662

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  13. pJH3456

    Plasmid
    #179581
    Purpose
    Pnmr-1::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 BFP
    Depositor
    Mei Zhen
    Article
    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1
    Insert
    tomm20::miniSOG (tomm-20 Nematode)
    Use
    Tags
    BFP
    Expression
    Worm
    Mutation
    Promoter
    Pnmr-1
    Available since
    March 2, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    179581-plasmid-map-sequence-id-355714

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  25. pSMOX-mNG::LacI-V3

    Plasmid
    #210224
    Purpose
    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.
    Depositor
    Calvin Tiengwe
    Article
    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115
    Insert
    mNeongreen Lac repressor fusion protein
    Use
    Trypanosoma brucei expression
    Tags
    Expression
    Mutation
    Promoter
    Pol II readthrough transcription after integration
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    210224-plasmid-map-sequence-id-415797

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  27. pAAV-TetON-CD4

    Plasmid
    #217537
    Purpose
    AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged CD4 receptor
    Depositor
    Peter Scheiffele
    Article
    Okur et al bioRxiv 2023
    Insert
    CD4 receptor (CD4 Human)
    Use
    AAV
    Tags
    HA epitope
    Expression
    Mammalian
    Mutation
    Promoter
    TetOperon (TetO)
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    217537-plasmid-map-sequence-id-429499

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  28. sgRNA3_GAL4UAS-Luciferase reporter

    Plasmid
    #64159
    Purpose
    Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette
    Depositor
    Moritoshi Sato
    Article
    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi
    Insert
    sgRNA3 for GAL4UAS-Luciferase reporter
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64159-plasmid-map-sequence-id-107444

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  50. pCAG-C-CreintG

    Plasmid
    #69573
    Purpose
    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells
    Depositor
    Connie Cepko
    Article
    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do
    Insert
    kozak consensus - C-CreintG
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    69573-plasmid-map-sequence-id-129736

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Showing: 10551 - 10600 of 28572 results